高效液相色谱法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定  

Uncertainty evaluation of determination of vitamin B_6 content in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography

作　　者：庞学斌[1] 张伟[1] 邱颖姮[1] 邬晓鸥[1] 

机构地区：[1]深圳药品质量标准研究重点实验室/深圳市药品检验所,广东518057

出　　处：《现代医药卫生》2015年第7期982-985,共4页Journal of Modern Medicine & Health

摘　　要：目的评定高效液相色谱法(HPLC法)测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度。方法采用HPLC法测定试样中维生素B6的含量,根据建测量模型,确定影响不确定度的分量,并加以评定量化,得出合成不确定度和扩展不确定度。结果试样中维生素B6含量测得值为0.498 mg/100 m L,其中B6含量的相对不确定度为0.024 9,标准不确定度为0.012 4 mg/100 m L,扩展不确定度为0.024 8 mg/100 m L(k=2)。结论建立的计算方法实用、可靠,可以用于HPLC法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定,经评定发现,高效液相色谱仪定量重复性对不确定度的影响最大。Objective Uncertainty evaluation of determination of vitamin B6 content in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography（HPLC assay）. Methods HPLC was adopted for the determination of vitamin B6 content. Mathematical model was established for the evaluation of uncertainty to quantize influential factors of uncertainty and evaluate the uncertainty factors further. Results The content of vitamin B6 in sample oral liquid was 0.498 mg/100mL,in which, the relative uncertaintyof vitamin B6 content was 0.024 9,the standard uncertainty was 0.0124 mg/100 mL,the expanded uncertainty was 0.024 8 mg/100 mL（k=2）. Conclusion The calculation method is practical and reliable,being access to the uncertainty eval—uation of content of vitamin B6 in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography. Upon the evaluation,it is found that the quantitative repetitive of the HPLC is the major impact.

关 键 词：色谱法 高压液相 维生素B6 牡蛎 数学计算 

分 类 号：R286.0[医药卫生—中药学]