应用大鼠血液置换模型对新型携氧代血浆功效的初步实验观察  

Replacement of rat blood with the new generation of multi-functional oxygen-carrying plasma

作　　者：闫东[1] 李遥金[1] 郝莎莎[1] 蒋冰玉[1] 李燊[1] 张学俊[1] 王红[1] 刘嘉馨[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2015年第3期260-263,共4页Chinese Journal of Blood Transfusion

基　　金：国家高技术研究发展计划(863计划)子课题:人源血红蛋白衍生物关键技术研究和扩张床技术在血浆蛋白纯化中的应用研究(2012AA021903-3);四川省技术创新工程专项"血液代用品--人源性血红蛋白衍生物关键科学技术研究"(2013ZZ0006);四川省国际科技合作与交流研究计划"基于人源性血红蛋白第三代血液代用品的联合开发"(2014HH0065)

摘　　要：目的制备1种羟乙基淀粉与血红蛋白载氧体(HBOC)相结合的多功能携氧代血浆,并对该携氧代血浆在大鼠血液置换模型中的有效性做初步观察。方法在无菌条件下将2种HBOC样品[Ⅰ(poly-Hb-1)和样品Ⅱ(poly-Hb-2)]各20 m L与羟乙基淀粉130按照相同的配方分别制备成携氧代血浆制品样品,2组制品中Hb均为2 g/d L。30只雄性SD大鼠随机均分为羟乙基淀粉130(HES130)、poly-Hb-1+HES130和poly-Hb-2+HES130等3组,大鼠换血后除饮水和饲喂外未采取其他治疗措施,并分别于换血前、换血过程末段及换血后1 h、24 h记录MAP、Hct等生理指标,取血测定动脉血血气及全血细胞计数,并考察换血≤72 h大鼠的生存质量和存活情况。结果 HES130组、poly-Hb-1+HES130组与poly-Hb-2+HES130组比较,换血末段的MAP(mm Hg)为99±4 vs 111±3 vs 117±5(P<0.05),Hct(%)为16±2 vs 19±1 vs 17±3(P<0.05);换血后1 h的Hct(%)为17±2 vs 20±3 vs 21±2(P<0.05);换血后24 h,Hct(%)为16±2 vs 19±3 vs 20±2(P<0.05),动脉血乳酸值(mmol/L)为7.1±1.2 vs 2.3±0.4 vs 1.2±0.2(P<0.05),动脉剩余碱(BE)(mmol/L)为-5.6±2.4 vs 1.5±0.7 vs 1.9±0.4(P<0.05);换血后1及24 h的RBC(×1012/L)分别为3.10±0.23 vs 3.33±0.34 vs 3.49±0.17与2.08±0.11 vs 2.38±0.17 vs 3.23±0.17(P<0.05);换血后1 h的WBC(×109/L)为6.10±1.04 vs 7.60±0.59 vs 7.39±0.54(P<0.05);换血后24、48、72 h存活率分别为50%vs 80%vs 100%、40%vs 60%vs 90%、30%vs 40%vs 80%。结论具有携氧功能的代血浆能有效维持换血模型大鼠的MAP,其缓解代谢性酸中毒效果优于仅有HES的大鼠换血模型;这种增加携氧功能以后的代血浆能明显改善大鼠存活质量,和现有胶体代血浆相比具有明显的优势。Objective To investigate the biological function of the new generation of multi-functional oxygen-carrying plasma,which combined hydroxyethyl starch and hemoglobin-based oxygen-carrying plasma,as a replacement for rat blood,.Methods Two kinds of HBOC samples poly-Hb-1 and poly-Hb-2 were taken from our laboratory,followed by the preparation of oxygen-carrying plasma substitute products with HES 130 under sterile conditions. Rats with two samples of hemoglobin concentration at 2 g / d L. 30 SD were randomized into three groups： HES130 group,poly-Hb-1 ＋ HES130 group and polyHb-2 ＋ HES130 group. Physiological parameters,blood gas parameters and blood cells counter were monitored without any treatment. The 72-hour survival rate of the rats was also observed. Results After transfusion,MAP（ mm Hg） of three sample groups were 99 ± 4 vs 111 ± 3 vs 117 ± 5（ P〈0. 05）,Hct（ %） were 16 ± 2 vs 19 ± 1 vs 17 ± 3（ P〈0. 05）. One hour after transfusion,Hct of three sample groups were 17 ± 2 vs 20 ± 3 vs 21 ± 2（ P〈0. 05）; 24 hours after transfusion,Hct of three sample groups were 16 ± 2 vs 19 ± 3 vs 20 ± 2（ P〈0. 05）. The arterial blood lactate（ mmol / L） were 7. 1 ± 1. 2 vs 2. 3± 0. 4 vs 1. 2 ± 0. 2（ P〈0. 05）. The arterial blood base excess parameters were-5. 6 ± 2. 4 vs 1. 5 ± 0. 7 vs 1. 9 ± 0. 4（ P〈0. 05）. One hour and 24 hours after transfusion,red blood cell counts（ × 10^12/ L） were 3. 10 ± 0. 23 vs 3. 33 ± 0. 34 vs 3. 49± 0. 17 and 2. 08 ± 0. 11 vs 2. 38 ± 0. 17 vs 3. 23 ± 0. 17（ P〈0. 05）. One hour after transfusion,white blood cell counts（ ×10^9/ L） were 6. 10 ± 1. 04 vs 7. 60 ± 0. 59 vs 7. 39 ± 0. 54（ P〈0. 05）. The 24-hour,48-hour and 72-hour survival rates of the HES130 group,poly-Hb-1 ＋ HES130 group and poly-Hb-2 ＋HES130 group were 50% vs 80% vs 100%,40% vs 60% vs90%,30% vs 40% vs 80%,respectively. Conclusion The new generation of multi-functional oxygen-carrying plasma can effectively maintain mean arterial pressure in

关 键 词：代血浆 羟乙基淀粉 血红蛋白载氧体 动物换血模型 复苏液 

分 类 号：R457.14[医药卫生—治疗学] R641[医药卫生—临床医学]