一氧化氮对保存悬浮红细胞部分功能的量效关系初步比较  

The primary contrast of nitric oxide dose-effect on partial function of stored RBC

作　　者：张警丹[1] 王红[1] 钟锐[1] 蒋冰玉[1] 韩玎玎 贺曾[1] 杨琨[1] 刘嘉馨[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2015年第3期271-276,共6页Chinese Journal of Blood Transfusion

摘　　要：目的确定2种一氧化氮(NO)药物在保存期间介入红细胞(RBC)的最佳浓度,从而为改善RBC保存质量提供依据。方法血样采自8名健康献血者,制成悬浮红细胞4℃保存,分别在不同保存时期取样,1)从同1份标本中取出7等份,前6份为实验组,3份加适宜浓度的硝普钠(SNP)溶液分为3个浓度梯度,另3份加L-精氨酸(Larg)分为3个相同的浓度梯度;最后1份对照组添加等体积的等渗PBS,7组一起置于室温下孵育1 h后检测RBC变形性和渗透脆性。2)将孵育后的RBC用Percoll细胞分离液,按平均密度大小分层依次吸取每层RBC并检测其功能。结果 1)保存初期、中期未分层RBC实验组变形性和渗透脆性,与对照组相比均无统计学差异(P>0.05)。保存末期未分层RBC 2项指标中,10μmol/L SNP和10μmol/L、100μmol/L L-arg与对照组均有统计学差异(P<0.05)。2)经密度梯度离心后,分离出4层红细胞,其中2、3、4层为可用的红细胞层,在保存初期、中期第2、3层RBC实验组与对照组无统计学差异(P>0.05),初期4层细胞2项指标在添加10μmol/L、100μmol/L SNP和10μmol/L L-arg后,与对照组细胞有统计学差异(P<0.05),中期4层细胞2项指标中10μmol/L、100μmol/L SNP和10μmol/L、100μmol/L、1 000μmol/L L-arg实验组,与对照组细胞有统计学差异(P<0.05);保存末期只有4层RBC的2项指标在分别介入100μmol/L SNP和100μmol/L L-arg后,与对照组均有统计学差异(P<0.05)。结论 NO只对老化和损伤RBC有改善效果,且保存初期、中期SNP、L-arg 2种药物的最佳浓度均为10μmol/L,保存末期2种药物的最佳浓度均为100μmol/L。Objective To determine the best concentration of NO drugs during the storage period,in order to provide a scientific basis for improving the storage quality of RBC. Methods Blood samples were first collected from eight healthy donors from Chengdu,centrifuged to make a suspension of RBC and then were stored at 4o C. Samples were extracted at different time duration of storage. 1） Seven moieties were divided from the same suspension of RBC： six parts were experimental groups（ among which,three were added different concentrations of SNP drug and were labeled as the SNP group,other three were added different concentrations of L-arg drug and were labeled as the L-arg group） and one part was the control group with the addition of the same volume of isotonic PBS. All seven groups were incubated at indoor temperature for one hour,then the deformability and osmotic fragility of RBC were determined. 2） RBC were separated using Percoll and extracted from each layer after incubation to elucidate the functions of the layers. Results 1） The prophase stage and metaphase of erythrocytes that had never been separated（ mixed RBC） displayed no significant difference between the experiment groups and the control group on the two testing indices（ P〈0. 05）. The mixed RBC exhibited significant difference between the experiment groups and the control group（ P〈0. 05） at the terminal stage. 2） After separation according to density gradient,cells were separated into four layers,of which only the second,third and fourth layer could be used. Both RBCs from the second and third layers showed no significant difference between the experiment groups and the control group（ P 〉0. 05） during prophase and metaphase. During prophase,the erythrocytes from the fourth layer with the addition of 10 μmol / L and 100 μmol /L of SNP and 10 μmol / L L-arg expressed significant differences than the control group on the two testing indices（ P 〈0. 05）. During metaphase,the experimental groups that showed significant

关 键 词：硝普钠 L-精氨酸 一氧化氮 悬浮红细胞 密度梯度离心 

分 类 号：R457.12[医药卫生—治疗学]