贵州地区随机人群血小板抗原基因频率调查和不配合率分析  

Investigation and analysis on gene frequency of platelet antigen and noncooperative rate in randomly drawn cases in Guizhou province

作　　者：尹红 杨芳[1] 杨晋渝[1] 曾小菁[1] 钟娜娜[1] 

机构地区：[1]贵阳医学院检验系,贵州贵阳550004 [2]息烽小寨坝中心卫生院

出　　处：《中国输血杂志》2015年第3期279-282,共4页Chinese Journal of Blood Transfusion

基　　金：贵州省科委资助基金(E2009-17)

摘　　要：目的研究贵州地区随机人群HPA-1—5,15基因多态性分布特点及不配合率分析,为本地区HPA免疫性疾病的防治和建立HPA资料库提供参考数据。方法采用PCR-SSP方法对本地区1 270例随机人群进行HPA-1—5,15系统基因分型、计算基因频率和等位基因不配合率,得出相关数据,并与国内外不同地区人群进行比较,阐明本地区特点。结果本地区HPA-1、2、4、5以aa基因型为主基因型,表型频率0.883-0.997,除8例HPA-2bb纯合子外,HPA-1、4、5未检出bb基因型;HPA-3和15高度杂合,表型频率分别为3aa=0.371、3ab=0.486、3bb=0.143、15aa=0.265、15ab=0.493、15bb=0.242。本地HPA各系统与其他地区比较差异显著的有,HPA-1、5高加索人、埃及和美国白人,HPA-2成都和埃及,HPA-3广州和青岛,HPA-4青岛,HPA-15与各地均无差异。不配合率由高到低依次为HPA-15=0.375、HPA-3=0.362、HPA-2=0.110、HPA-5=0.051、HPA-1=0.012、HPA-4=0.004。结论本地区随机人群HPA-1-5、15系统1、2、4和5以aa基因型为主,3和5高度杂合,呈多态性分布。在本地区应该高度警惕HPA-3和15系统同种抗体导致的PTP、PAT和PTR的可能性,其次为HPA-2和5,由HPA-1和4系统产生同种免疫的机率较低。本研究证实HPA多态性分布存在人群、地域和人种的差异。Objective To study the distribution characteristics of gene polymorphism,to analyze the noncooperative rate of HPA-1—5 and 15 in the population randomly drawn in Guizhou province,and to provide evidence based knowledge to guide prevention strategies for HPA autoimmune disease and the establishment of a HPA database. Methods PCR-SSP was adopted to genotype the HPA-1-5 and 15 system in 1,270 randomly drawn cases. Gene frequency and noncooperative rate of the population in Guizhou were calculated and were compared with those living in different regions domestically and internationally. Lastly,regional characteristics were elucidated. Results The primary genotype of HPA-1,2,4,5 in this region was aa genotype. The phenotypic frequency of aa was approximately 0. 883- 0. 997. Excluding the 8 cases that were homozygous for bb in HPA-2,bb genotype was not noted in HPA-1,4,5 system. The HPA-3 and 15 were predominated by heterozygotes. Phenotypic frequencies for 3aa,3ab,3bb,15 aa,15ab and 15 bb were 0. 371,0. 486,0. 143,0. 265,0. 493 and 0. 242,respectively. Comparing the HPA system in this region and other domestic and international regions,significant differences were found in the HPA-1,5 from Caucasians,Egyptians and Caucasian Americans,HPA-2 from Chengdu and Egypt,HPA-3 from Guangzhou and Qingdao,HPA-4 from Qingdao. No difference was found in HPA-15 in all regions. The noncooperative rate was ranked from high to low in the descending order： HPA-15 = 0. 375,HPA-3 = 0. 375,HPA-2 =0. 110,HPA-5 = 0. 051,HPA-1 = 0. 012,and HPA-4 = 0. 004. Conclusion The aa genotype is given priority among the HPA-1,2,4 and 5 in the HPA-1-5 and 15 systems. HPA-3 and 5 were highly heterozygous,exhibiting a polymorphic distribution. In this region,attention should be paid to the HPA-3 and 15 systems which are likely to cause PTP,PAT and PTR,and also HPA-2 and 4 and HPA-1 and 4 systems which have low probability to produce same immune responses. This study confirmed that the the distribution of HPA polymorphism is different in varying popula

关 键 词：贵州地区 人类血小板抗原 基因多态性 PCR-SSP 

分 类 号：R331.143[医药卫生—人体生理学] R457.11[医药卫生—基础医学]