桂竹糖芥苷对大鼠肾髓袢升支粗段管周膜50 pS钾通道活性的调控机制  

The regulatory mechanism of erysimosides on the basolateral 50 pS potassium channel in the thick ascending limb

作　　者：隋洪玉[1] 齐淑芳[1] 贾秀月[1] 王毅 

机构地区：[1]佳木斯大学生理教研室,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2015年第2期38-39,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省卫生厅科研项目;编号:2012-250;佳木斯大学青年基金资助课题;编号:Sq2012-36

摘　　要：目的:探讨桂竹糖芥苷对肾髓袢升支粗段(thick ascending limb,TAL)管周膜50 p S钾通道活性的调控机制。方法:采用细胞贴附式膜片钳技术及免疫印迹技术测定桂竹糖芥苷对50 p S钾通道活性的调控机制。结果:桂竹糖芥苷可明显抑制50 p S钾通道的活性(P<0.01);PKC阻断剂存在时,桂竹糖芥苷不再增加50 p S钾通道的活性(P>0.05)。结论:桂竹糖芥苷可能是通过激活PKC依赖途径抑制50 p S钾通道活性的。Objective： To study the regulatory mechanism of erysimosides on the 50 p S potassium channel in the thick ascending limb（ TAL） of rat kidney. Methods： The patch- clamp cell- attached recording technique was used to record the single potassium channel current. The western blot technique was used to detect the protein express of phosphate- PKC. Results： Erysimosides can decrease the 50 p S potassium channel activity of TAL,and PKC inhibitor can inhibit the effect. Conclusion： Erysimosides can inhibit the activity of basolateral 50 p S potassium channel by the PKC- dependent pathway.

关 键 词：桂竹糖芥苷 髓袢升支粗段 钾通道 PKC 

分 类 号：R285.1[医药卫生—中药学]