外周血Th17和CD_4^+ CD_(25)^+调节性T细胞变化与患儿支气管哮喘活动状态的相关性研究  

Correlation between State of Bronchial Asthma Children and Peripheral Blood Th17 and CD_4^+ CD_(25)^+ Regulatory T Cells Changes

作　　者：陈啸洪[1] 李华浚[1] 姚欢银[1] 朱芹[1] 张佩红[1] 章杭湖[1] 刘小群[1] 周国中[2] 

机构地区：[1]浙江省绍兴市人民医院儿内科,312000 [2]浙江省绍兴市人民医院临床检验中心,312000

出　　处：《中国全科医学》2015年第8期969-971,共3页Chinese General Practice

基　　金：绍兴市科技计划项目(2012B70069)

摘　　要：目的探讨外周血Th17和CD+4CD+25调节性T细胞(CD+4CD+25Treg)变化与患儿支气管哮喘活动状态的相关性。方法选取2010年1月—2013年12月于绍兴市人民医院哮喘专科门诊就诊的慢性持续期支气管哮喘患儿98例为慢性持续期组,选择同期在本院治疗的缓解期支气管哮喘患儿40例为缓解期组,选择同期在本院儿童保健门诊体检健康的儿童40例作为健康对照组。采用流式细胞仪测定受试者外周血中Th17及CD+4CD+25Treg。结果三组间Th17、CD+4CD+25Treg和Th17/CD+4CD+25Treg比较,差异均有统计学意义(F值分别为5.274、7.813和3.526,P<0.05)。Th17、Th17/CD+4CD+25Treg与儿童哮喘控制测试量表(C-ACT)评分呈正相关(r值分别为0.823、0.485,P<0.05),CD+4CD+25Treg与C-ACT评分呈负相关(r=-0.562,P<0.05),Th17与CD+4CD+25Treg呈负相关(r=-0.273,P<0.05)。结论慢性持续期支气管哮喘患儿Th17免疫应答增强,CD+4CD+25Treg免疫功能下降,支气管哮喘的活动状态与外周血中的Th17/Treg细胞免疫失衡密切相关。Objective To investigate the correlation of the active state of asthma with the peripheral blood Th17 and CD＋4CD＋25regulatory T cells（ Treg） change on children. Methods From January 2010 to December 2013,98 cases of children with asthma in Shaoxing People＇s Hospital specialist outpatient clinic were selected for chronic duration group,meanwhile 40 cases of remission asthma children in the same hospital were selected as remission group. 40 cases of healthy children in child health clinic were selected as healthy control group. Using flow cytometry to measure children＇s peripheral blood Th17 and CD＋4CD＋25Treg. Results In three groups comparison,there were significant differences among Th17,CD＋4CD＋25Treg and Th17 /CD＋4CD＋25Treg（ F = 5. 274,7. 813,3. 526, P 0. 05）. There was positive relationship between the score of Th17 cells,Th17 / CD＋4CD＋25Treg and C- ACT（ r = 0. 823,0. 485,P 0. 05）. CD＋4CD＋25Treg was negatively related with C- ACT（ r =-0. 562,P 0. 05）. Th17 cells was negatively related with CD＋4CD＋25Treg（ r =- 0. 273,P 0. 05）. Conclusion Children with chronic asthma duration,Th17 immune response enhanced,and CD＋4CD＋25Treg immune function declined,the severity of asthma and peripheral blood Th17 / Treg of the immune imbalances are closely related.

关 键 词：哮喘 T淋巴细胞 辅助诱导 T淋巴细胞 调节性 儿童 

分 类 号：R725.622.5[医药卫生—儿科]