贵州6个白刺花种群种子贮藏蛋白的遗传多样性  

Genetic Diversity of Seed Storage Protein of Six Sophora davidii Populations in Guizhou

作　　者：马林[1] 王普昶 姚红艳[1] 赵丽丽[1] 陈超[1] 卢立娜 

机构地区：[1]贵州大学动物科学学院草业科学系,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550006 [3]鄂尔多斯市林业治沙科学研究院,内蒙古东胜017000

出　　处：《贵州农业科学》2015年第3期5-8,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"贵州石漠化功能灌木群繁殖行为与种群自组织更新响应机制"(31260572);贵州省科教青年英才培育工程项目"贵州白刺花种群遗传变异及指纹图谱构建"[黔省专合字(2012)142];贵州省农业攻关项目"雀稗属牧草无融合生殖固定杂种优势研究与示范"[黔科合NY(2014)3048];贵州省农业攻关项目"灌丛草地改良及配套养羊关键技术研究与示范"[黔科合NY字(2010)3049]

摘　　要：为探明贵州白刺花的遗传背景及其种质资源的开发利用提供理论依据，采用十二烷基硫酸钠－聚丙烯酰胺凝胶（SDS－PAGE）电泳对采自贵州不同海拔梯度下6个白刺花种群的种子贮藏蛋白，构建6个种群的种子贮藏蛋白指纹图谱，并进行聚类分析。结果表明：6个白刺花种群的种子贮藏蛋白总条带数为22条，分子量在17．97～81．91 kDa，其中多态性条带8条，多态性百分率为36．36％。SDS—PAGE 电泳图可作为白刺花的生化指纹，各种群种子贮藏蛋白电泳图上具有各自的特征带或缺失带。相邻高海拔的白刺花种群间遗传差异相对较小，先聚为一类，与低海拔种群遗传差异较大，最后聚为一类。The fingerprint spectrums of seed storage protein of six S.davidii populations with different elevation were established by SDS-PAGE to probe genetic background of S.davidii and provide the theoretical basis for development and utilization of S.davidii germplasm resources.The results showed that 22 bands of seed storage protein with 17.97～81.91 kDa molecular weight were detected from 6 S.davidii populations,and eight bands are of polymorphism.SDS-PAGE electrophoretogram can be used as the biochemical fingerprint of S.davidii and there are own characteristics bands and deficiency band in seed storage protein electrophoretogram of different S.avidii population.The cluster analysis showed that the genetic difference of adjacent high-altitude S.davidii populations were relatively smaller and there is great genetic difference between S.davidii populations with high altitude and S.davidii populations with low altitude.

关 键 词：白刺花 种子贮藏蛋白 指纹图谱 聚类分析 贵州 

分 类 号：S514[农业科学—作物学]