反转录-环介导等温扩增技术(LAMP)快速检测马铃薯病毒  

Rapid Detection of PVX,PVY and PLRV by Reserve Transcription Loop-mediated Isothermal Amplification(RT-LAMP)

作　　者：陈恩发 李其义 邓宽平 卢扬 彭慧元 

机构地区：[1]贵州省生物技术研究所,贵州贵阳550006 [2]贵州省种子管理站,贵州贵阳550002

出　　处：《贵州农业科学》2015年第3期74-77,共4页Guizhou Agricultural Sciences

基　　金：贵州省农业科学院专项资金项目"贵州马铃薯晚疫病数字防控技术研究与应用"[黔农科院院专项(2013)007];贵州省科技厅农业攻关项目"马铃薯微型薯环保型种衣剂研究与应用"[黔科合NY字(2010)3012];贵州省国际科技合作项目"马铃薯晚疫病抗性材料引进与田间评价"[黔科合外G字(2012)7026]

摘　　要：为寻求低成本快速准确灵敏度高的马铃薯病毒检测方法,根据马铃薯X病毒(Potato virus X,PVX)、马铃薯Y病毒(Potato virus Y,PVY)和马铃薯卷叶病毒(Potato leafroll virus,PLRV)的外壳蛋白基因序列设计4条特异引物,建立LAMP检测技术。结果表明:LAMP检测方法灵敏度下限是10个拷贝的病毒量,该方法检测PVX、PVY和PLRV特异性好,灵敏度高,1h可完成检测,且检测成本低。RT-Lamp detection technology was established by designing four specific primers according to coat protein gene sequences of PVX,PVY and PLRV to probe potato virus detection method with lower cost,rapidity,accuracy and high sensitivity.The results showed that the lower limit of sensitivity of RT-LAMP detection method is virus count of 10 copies.PVX,PVY and PLRV can be successfully detected by the established RT-LAMP detection method with lower cost,rapidity,accuracy and high sensitivity in 1 h.

关 键 词：马铃薯X病毒 马铃薯Y病毒 马铃薯卷叶病毒 反转录-环介导等温扩增技术 (RT-LAMP) 病毒检测 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]