贵州地区土壤中需氧放线菌的分离鉴定  

Isolation and Identification of Aerobic Actinomycetes in Soil in Guizhou

作　　者：金方[1] 刘涛华[1] 牟丽丽[1] 吕倩[1] 王颜颜[1] 康颖倩[1] 

机构地区：[1]贵阳医学院微生物学教研室,贵州贵阳550004

出　　处：《贵州农业科学》2015年第3期114-117,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金地方项目"贵州省病原需氧放线菌的系统分类研究及其属间快速鉴别诊断试剂盒的研发"(31260029);贵州省国际科技合作计划项目"白酒丢糟及锅底水水解循环利用技术"[黔科合外G字(2014)7006];贵州省社会发展科技攻关项目"贵州省放线菌资源的开发及临床放线菌属间快速鉴别诊断试剂盒的研发"[黔科合SY字(2011)3017];贵阳市科技局科技创新平台计划项目"姜黄纳米保健产品科技创新平台的建设"[筑科合同2012303]

摘　　要：为了解贵州土壤需氧放线菌的分布特点、系统发育和物种多样性,采集贵州贵阳、遵义、安顺、铜仁、毕节、黔南、六盘水、黔东南、黔西南9个区域的农耕地、湿地、森林3个类型土壤标本进行需氧放线菌的分离纯化,扩增其16SrDNA基因序列后在GenBank中进行Blast比对分析,并以邻接法构建系统发育树。结果表明:在贵州9个区域108份土壤中分离出173株需氧放线菌,其中链霉菌属(Streptomyces)165株、北里孢菌属(Kitasatospora.)5株、诺卡氏菌属(Nocardioides)2株,在遵义农耕地土壤中分离出链霉菌属潜在新种1株(GYM8-3)。贵州土壤需氧放线菌主要为链霉菌属,在种间水平上存在分类多样性和地域差异性。The aerobic actinomycetes of soil samples from farmland,wet land and forest of Guiyang, Zunyi,Anshun,Tongren,Bijie,South Guizhou,Liupanshui,Southeast Guizhou and Southwest Guizhou were isolated and purified,then their amplified 16S rDNA gene sequences were compared with GenBank and finally the phylogenetic tree was established by the neighbor-joining method to know distribution feature,phylogeny and species diversity of soil aerobic actinomycetes in Guizhou.The results showed that 173 aerobic actinomycete strains including 165 Streptomyces strains,five Kitasatospora strains,two Nocardioides strains and 1 new potential Streptomyces strain are isolated from 108 soil samples.Soil aerobic actinomycetes belong to Streptomyces and there are classification diversity and regional difference at the species level.

关 键 词：需氧放线菌 链霉菌 土壤 贵州 

分 类 号：S154.3[农业科学—土壤学] Q939[农业科学—农业基础科学]