传染性法氏囊病病毒衣壳蛋白与宿主CEF细胞相互作用蛋白的筛选  

Screening of CEF Cellular Proteins Interacting With Capsid Protein of Infectious Bursal Disease Virus

作　　者：陈玉明[1] 高立[1] 王念[1] 张礼洲[1] 卢珍[1] 高玉龙[1] 王永强[1] 高宏雷[1] 李凯[1] 刘长军[1] 崔红玉[1] 张艳萍[1] 王笑梅[1,2] 祁小乐[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001 [2]江苏省动物重要疫病与人畜共患病协同创新中心,江苏扬州225009

出　　处：《中国家禽》2015年第7期16-19,共4页China Poultry

基　　金：国家自然科学基金(31430087);现代农业产业技术体系建设专项基金(nycytx-42-G3-01);哈尔滨市应用技术研究与开发基金(2014AB3AN058)

摘　　要：为筛选与传染性法氏囊病病毒(IBDV)衣壳蛋白VP2相互作用的宿主细胞蛋白,本研究以鸡胚成纤维细胞(CEF)为宿主细胞模型,VP2蛋白为诱饵,采用MatchmakerTMGold系统,进行酵母双杂交试验。结果显示杂交效率为15%,所筛选的酵母总数为7.5×106个。回转验证等试验表明,有4种宿主细胞蛋白可以与VP2蛋白发生相互作用,本研究为深入研究IBDV的致病机制奠定了基础。This study was conducted to screen host cellular proteins that interacted with capsid protein VP2 of infectious bursal disease virus（IBDV）. For this purpose,with MatchmakerTMGold system,VP2 protein was used as bait in the yeast two- hybrid assay to screen the interactive proteins from chicken embryo fibroblast（CEF）. The results showed that the mate efficiency was 15% and the total number of yeast screened was 7.5×106. With co-transformation and other experiments,four host cellular proteins were confirmed to interact with VP2 protein. The research contributes substantially to better studying the pathogenic mechanism of IBDV.

关 键 词：传染性法氏囊病病毒 衣壳蛋白 宿主细胞蛋白 酵母双杂交 相互作用 

分 类 号：S855.3[农业科学—临床兽医学]