薄荷抗单纯疱疹病毒有效部位筛选研究  

Reseach on Screening of Effective Antiviral Parts of Mentha Haplocalyx

作　　者：陈飞[1] 姚梅悦[1] 张霞[1] 周长征[2] 

机构地区：[1]山东中医药大学 [2]山东中医药大学药学院,山东济南250355

出　　处：《山东中医杂志》2015年第4期289-291,共3页Shandong Journal of Traditional Chinese Medicine

摘　　要：目的 :用大孔树脂吸附法提取薄荷的12个化学部位进行体外抗单纯疱疹病毒(HSV-1)研究,以确定薄荷抗单纯疱疹病毒的具体有效部位。方法:把薄荷的水煎液浓缩至一定体积上样于大孔吸附树脂柱,分别用水、25%乙醇、50%乙醇、75%乙醇进行洗脱,采用HSV-1-Hep-2(其中Hep-2为人喉癌上皮细胞)细胞体外病毒感染模型,进行体外抗HSV-1作用评价,通过显微镜下观察细胞病变效应(CPE),以酶标仪测定中性红染色A540值,计算治疗指数(TI)。结果:薄荷大孔树脂水洗脱液2号具有显著的抗HSV-1活性,其TI指数为74.3。结论:薄荷水溶性成分对HSV-1病毒有明显的抑制作用。Objective：To determine the effective anti-herpes simplex virus（HSV） part of mentha haplocalyx by anti-HSV research in vitro on 12 chemical parts of mentha haplocalyx extracted by macroporous resin adsorption. Methods：The water decoction of mentha haplocalyx was concentrated to certain volume and the samples was put on the macropore adsorption resin. The samples was washed by water,25% ethanol,50%ethanol and 75% ethanol. The in vitro effect of anti HSV-1 was evaluated by in vitro infection model of HSV-1-Hep 2 cells. The cytopathic effect（CPE） was observed by microscope,the neutral red staining A540 value was determined by ELIASA and the therapeutic index（TI）was calculated. Results：The NO.2 water elution has remarkable anti HSV-1 effect and the TI was 74.3. Conclusion：The water soluble ingredients of mentha haplocalyx has remarkable intibitory effect on HSV-1.

关 键 词：薄荷 有效部位 大孔树脂 单纯疱疹病毒 抗病毒 实验研究 

分 类 号：R284.2[医药卫生—中药学]