血小板膜糖蛋白CD61、CD62P在小鼠脓毒症炎性反应中的调控作用  

Regulatory role of the platelet membrane glycoprotein CD61 and CD62P in septic inflammation

作　　者：孙运秀[1] 薛寅凯 刘延军[2] 朱鹏程[2] 窦东伟[2] 陈立波[2] 程平[2] 杨东[3] 

机构地区：[1]武汉市中心医院急诊外科,430014 [2] 华中科技大学同济医学院附属协和医院急诊创伤外科 [3] 华中科技大学同济医学院附属协和医院麻醉与危重医学研究所

出　　处：《中华实验外科杂志》2015年第4期745-747,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察血小板膜糖蛋白CD61,CD62P在脓毒症小鼠炎性反应中的调控作用.方法 采用盲肠结扎穿孔术（CLP）制作小鼠脓毒症模型;将60只BALB/c雄性小鼠随机分为阴性对照组、假手术组、CLP组、CLP＋血小板膜糖蛋白受体拮抗剂替罗非班组（替罗非班组）.检测处理后24 h外周血血小板CD61、CD62P和血清肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6的表达.结果 CLP组血小板计数为（167.0 ±31.4）×106/L较阴性对照组[（289.7 ±45.9）×106/L]、假手术组[（267.7±25.4）×106/L]、替罗非班组[（217.0 ±29.7） ×106/L]明显降低（P＜0.01）;CLP组CD61、CD62P[（91.22 ±2.96）％、（7.87±2.90）％]及TNF-α、IL-6[（723.51±96.65）、（757.35±131.48） ng/L]表达较对照组明显升高（P＜0.01）,替罗非班组CD61、CD62P[（67.50±8.67）％、（3.53±0.78）％]及TNF-α、IL-6[（426.38±16.69）、（482.32±56.73）ng/L]表达较CLP组降低（P＜0.01）;脓毒症小鼠血小板膜糖蛋白CD61、CD62P表达与炎性因子的表达呈正相关.结论 血小板膜糖蛋白CD61、CD62P可能通过调控炎性因子表达参与脓毒症炎性反应的病理生理过程.Objective To observe the regulation of platelet membrane glycoprotein CD61,CD62P in septic inflammation.Methods Make the model of sepsis by cecal ligation and puncture （CLP）.Sixty male BALB/c mice were randomly divided into 4 groups （negative control,sham operation,CLP,CLP ＋ Tirofiban）.The platelet counts,expressions of CD61 and CD62P in peripheral platelets,levels of tumor necrosis factor-α （TNF-α） and interleukin （IL）-6 in serum were measured at 24 h after operation.Resuits Compared with those of negative control group [（289.7 ± 45.9） × 106/L],sham operation group [（267.7 ±25.4） × 106/L] and CLP ＋ Tirofiban group [（217.0 ± 29.7） × 106/L],the platelet count was lower in mice with CLP [（167.0 ±31.4） × 106/L,P 〈0.01].The expressions of CD61 [（91.22 ± 2.96）%],CD62P [（7.87 ±2.90）%],TNF-α [（723.51 ±96.65） ng/L] and IL-6 [（757.35 ± 131.48） ng/L] in mice with CLP increased significantly （P 〈 0.01）.However,the expressions of CD61 [（67.50±8.67）%],CD62P [（3.53 ±0.78）%],TNF-α [（426.38 ± 16.69） ng/L] and IL-6 [（482.32 ±56.73） ng/L] in mice with CLP ＋ Tirofiban were all lower than that of CLP group （P〈 0.01）.There is positive correlation between the expression of platelet membrane glycoprotein and the expression of inflammatory factors in mice with CLP.Conclusion Platelet activation is closely related to the inflammatory response of sepsis.Down-regulation of platelet membrane glycoprotein can significantly attenuate the septic inflammation.

关 键 词：脓毒症 血小板膜糖蛋白 CD61 CD62P 炎性反应 

分 类 号：R459.7[医药卫生—急诊医学]