微小RNA-29b对心肌缺血再灌注损伤的保护作用及机制  

The protective effect and mechanism of microRNA-29b on myocardial ischemia reperfusion injury

作　　者：魏远福[1] 江海[1] 邓毛[1] 刘敏[1] 朱立鑫[1] 王波[1] 

机构地区：[1]湖北医药学院附属人民医院心胸血管外科,十堰442000

出　　处：《中华实验外科杂志》2015年第4期811-814,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨微小RNA（miRNA,miR）-29b对心肌缺血再灌注损伤的保护作用及机制.方法 将成年雄性Wister大鼠40只随机分为假手术组、缺血再灌注损伤组、miR-29b治疗组和缺血再灌注阴性对照组4组.假手术组大鼠,放置6-0缝合线不结扎,其余3组均按缺血再灌注模型处理,其中miR-29b治疗组在缺血再灌注模型前24 h,心肌内注射agomiR-29b（1×103 mol/L）,阴性对照组在缺血再灌注模型前24 h,心肌内注射阴性对照序列.监测各组大鼠术后1、3、7、15、30 d各时相点的心率、左室射血分数（LVEF）、心肌细胞凋亡率、Ⅰ型胶原蛋白及miR-29b的mRNA表达.结果 各组大鼠术后1、3、7、15、30 d各组时相点心率差异无统计学意义（P＞0.05）;与假手术组比较,缺血再灌注组大鼠术后1、3、7、15、30 d左室射血分数（LVEF）分别为（70.42±2.06）％、（64.37±1.84）％、（54.61±1.37）％、（51.92±1.07）％、（47.38±1.02）％,LVEF变化均显著下降;与缺血再灌注组比较,miR-29b治疗组大鼠术后1、3、7、15、30 d心肌LVEF分别为（80.62±2.48）％、（78.37±2.03）％、（72.15±2.34）％、（65.18±1.73）％、（60.08±1.16）％,LVEF变化均显著上调;阴性对照组与缺血再灌注组比较差异无统计学意义（P＞0.05）.与假手术组比较,缺血再灌注组大鼠术后1、3、7、15、30 d心肌细胞凋亡率分别为（33.6±4.2）％、（53.7±6.3）％、（41.9±5.8）％、（39.3±6.7）％、（32.7±4.8）％,心肌细胞凋亡率均显著升高;与缺血再灌注组比较,miR-29b治疗组大鼠术后1、3、7、15、30 d心肌细胞凋亡率分别为（18.2±3.9）％、（29.6±5.2）％、（32.7±3.8）％、（28.4±6.0）％、（21.5±4.6）％,心肌细胞凋亡率均显著下降;阴性对照组与缺血再灌注组间各指标比较差异无统计学意义（P＞0.05）.与假手术组比较,缺血再灌注组大鼠术后各时相点Ⅰ型胶原蛋白表达均显著升高;而与缺�Objective To discuss the effect and mechanism of microRNA （miR）-29b on the myocardial ischemia-reperfusion （IR） injury.Methods Healthy adult male Wistar rats were divided into four groups：sham group,IR group,miR-29b group and scramble group.In sham group,6-0 linea suturalis was placed without deligation,and in the rest three groups IR model was maded.Rats in miR-29b group were given agomiR-29b （1 × 103 mol/L） at 24 h before IR modelling,and those in scramble group were given agomiR-29b （1 mmol/L） negative control sequence.Heart rate,left ventricular ejection frac-tion （LVEF）,apoptosis rate of myocardial cells,collagen Ⅰ and miR-29b at 1,3,7,15 and 30 d after operation were observed.Results Heart rate in all groups showed no significant difference at 1,3,7,15 d,and 30 d.Compared with sham group,I/R rats showed decreased LVEF,as （70.42 ± 2.06） %,（64.37±1.84）%,（54.61 ±1.37）%,（51.92±1.07）%,（47.38±1.02）% at 1,3,7,15,30 d,respectively.Compared with I/R group,LVEF in miR-29b group were significandy increased,as （80.62 ± 2.48）%,（78.37 ±2.03）%,（72.15 ±2.34）%,（65.18 ±1.73）%,（60.08 ±1.16）% at 1,3,7,15,30 d,respectively.There was no significant difference between I/R group and scramble group.Compared with sham group,I/R rats showed increased apoptosis rates of myocardial cell,as （33.6 ± 4.2） %,（53.7 ±6.3）%,（41.9 ±5.8）%,（39.3 ±6.7）%,（32.7 ±4.8）% at 1,3,7,15,30 d,respectively.Compared with I/R group,apoptosis rates of myocardial cell in miR-29b group were significantly decreased,as （18.2±3.9）%,（29.6±5.2）%,（32.7±3.8）%,（28.4±6.0）%,（21.5±4.6）% at 1,3,7,15,30 d,respectively.There was no significant difference between I/R group and scramble group.Compared with sham group,I/R rats showed increased collagen Ⅰ.Compared with I/R group,collagen Ⅰ in miR-29b group were significantly decreased.There was no significant difference between I/R group and scramble group.Compared with sham group,I/R rats showed decrea

关 键 词：心肌缺血 再灌注损伤 心室重塑 

分 类 号：R965[医药卫生—药理学]