急性子不同提取部位对人膀胱癌EJ细胞株生长的抑制作用  

Inhibitory effect of different fractions of impatiens balsamina L on proliferation of human bladder cancer cell line EJ

作　　者：蔡阳[1] 宋文[1] 丁玉峰[2] 杨俊[1] 陈瑞宝[1] 刘卓[1] 杨竣[1] 王涛[1] 王晨[2] 刘继红[1] 叶章群[1] 

机构地区：[1] 华中科技大学同济医学院附属同济医院泌尿外科 泌尿外科研究所,武汉430030 [2] 华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《中华实验外科杂志》2015年第4期818-820,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察急性子不同提取部位对人膀胱癌EJ细胞株生长的抑制作用.方法 将 急性子进行提取分离,得到水部、醇部、石油醚部、乙酸乙酯部及正丁醇部,通过细胞计数试剂盒（CCK-8）法检测各提取部位对人膀胱癌EJ细胞株增殖的抑制作用;流式细胞仪分析细胞周期分布及细胞凋亡.结果 急性子乙酸乙酯部、正丁醇部、水部、醇部、石油醚部对EJ细胞增殖均有一定抑制作用,呈剂量依赖关系,其对EJ细胞半数抑制浓度（IC50）依次为0.041、0.442、2.224、1.254、1.671 g/L.流式细胞仪检测结果显示,0.08 g/L的急性子乙酸乙酯部作用于EJ细胞24 h后,G2/M期细胞比例为（22.3±3.1）％,较空白对照组的（14.5±2.5）％显著增高（P＜0.05）.不同浓度的乙酸乙酯部作用于EJ细胞24h后,EJ细胞的凋亡率由对照组的（14.27±1.42）％分别增至（29.88±1.29）％、（36.91±2.35）％、（43.77±3.48）％、（77.73±3.18）％和（80.35±5.41）％.结论 急性子不同提取部位对人膀胱癌EJ细胞株的增殖均有一定抑制作用,其中乙酸乙酯部抑制作用最强,其机制可能与抑制细胞分裂和诱导细胞凋亡有关.Objective To investigate the inhibitory effect of different fractions of impatiens balsamina L on bladder cancer cell line EJ.Methods Impatiens balsamina L was extracted and separated to get water extracts,Ethanol extracts,oil ether extracts,ethyl acetate extracts and butanol extracts.Cell counting kit-8 （CCK-8） assay was used to detect the inhibitory effect of different fractions of impatiens balsamina L on human bladder cancer cell line EJ.Apoptosis rates and cell cycle distribution were detected by flow cytometry analysis.Results Ethyl acetate extracts,butanol extracts,water extracts,ethanol extracts and oil ether extracts could inhibited the proliferation of human bladder cancer cells EJ in a dose-dependent manner,the inhibition rate of proliferation was 0.041,0.442,2.224,1.254,1.671 g/L.After treated with 0.08 g/L Ethyl acetate extracts for 24 h,the percentage of G2/M was （22.3 ±3.1）%,significantly higher than the control group （14.5 ± 2.5） % （P 〈 0.05）.After treated with different concentrations Ethyl acetate extracts for 24 h,the percentage of apoptosis were （14.27 ± 1.42） %,（29.88 ± 1.29）%,（36.91 ±2.35）%,（43.77 ±3.48）%,（77.73 ±3.18）% and （80.35 ±5.41）% separately.Conclusion Impatiens balsamina L inhibited the proliferation of EJ cells,Ethyl acetate extracts have the strongest effect.Its mechanism might be associated with inducing cell apoptosis and stagnation at G2/M phase.

关 键 词：急性子 膀胱癌 细胞计数试剂盒 细胞周期 凋亡 

分 类 号：R285[医药卫生—中药学]