超短程化疗治疗脊柱结核外周血基因表达的研究  

Study of ultra-short-course chemotherapy treatment of spinal tuberculosis gene expression in peripheral blood

作　　者：牛宁奎[1] 王骞[2] 施建党[1] 王自立[1,3] 耿广起[1] 金卫东[1] 

机构地区：[1] 宁夏医科大学总医院脊柱外科,银川750004 [2] 美国南佛罗里达大学药学院 [3]生物芯片北京国家工程研究中心宁夏分中心

出　　处：《中华实验外科杂志》2015年第4期913-915,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81260282）;宁夏自然科学基金资助项目（NZ11195、NZ11275）

摘　　要：目的 探讨超短程化疗治疗脊柱结核临床疗效与外周血mRNA基因表达谱的关系.方法 实验组为27例脊柱结核患者,分为3组：A组（未治疗时段组）8例、B组（治疗结束时段组）9例、C组（1年随访时段组）10例.对照组为5例非结核患者.利用基因芯片筛选外周血差异表达基因,并对外周血炎性细胞因子及核转录因子（NF）-κB检测.结果 临床结果显示A组结核病灶活动;B、C两组结核病灶治愈.基因表达谱提示实验组、对照组有1 895个差异有统计学意义的基因;A、B组之间显著差异表达基因942个,上调936个,下调6个,这些基因主要参与炎症及宿主的免疫调节机制;B、C组之间表现为30条非特异性差异基因.A组较B、C两组肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-y）、白细胞介素-12（IL-12）、白细胞介素-8（IL-8）表达水平明显升高,这与NF-κB的表达水平相一致.结论 超短程化疗治疗脊柱结核的临床疗效与外周血mRNA基因表达水平相一致;外周血mRNA表达谱芯片可用于评估超短程化疗疗效.Objective Evaluate the relationship between the clinical efficacy of spinal tuberculosis using ultra-short-course chemotherapy （UCCT） and peripheral blood messenger RNA （mRNA） gene expressions.Methods The study group was composed of peripheral blood samples from 27 spinal TB patients,and it was divided into three groups：group A （untreated group） 8 cases,group B （treatment-completed group） 9 cases,and group C （1-yr follow-up group） 10 cases.The control group was made up of the peripheral blood samples from 5 non TB patients.Gene chip was employed to detect the differentially expressed gene in the peripheral blood samples.Detect the peripheral inflammatory cytokines and nuclear transcription factor κB （NF-κB）.Results Clinical result showed that the tuberculosis lesions in group A were active; while tuberculosis lesions in both group B and group C were all cured.The mRNA expression profiles in peripheral blood showed there were 1895 differentially expressed genes between study group and control group.However,there were 942 differentially expressed genes between group A and B,936 genes up-regulated and 6 genes down-regulated,and the functions of these differentially expressed genes were mainly related to the immune regulation of patients.No differential expressed genes were identified between group B and C,and they were non-specific differences 30 genes.Group A compared with group B and group C showed that tumor necrosis factor （TNF-α）,interferon-gamma （IFN-γ）,interleukin-12 （IL-12）,and interleukin-8 （IL-8） expression levels were significantly increased,which were consistent with the level of expression of NF-κB.Conclusion The clinical efficacy of UCCT for spinal tuberculosis was consistent with peripheral blood gene expressions,protein expression of inflammatory cytokines,and the level of NF-κB; mRNA gene expression profiles can be used to assess the efficacy of UCCT.

关 键 词：脊柱结核 外周血 基因表达谱 

分 类 号：R529.2[医药卫生—内科学]