O型口蹄疫病毒衣壳酸敏感性位点的筛选  

Selection of the Acid Sensitive Sites of FMDV Type O Capsid

作　　者：解银丽[1] 马琪[1] 秦晓东[1] 殷相平[1] 柳纪省[1] 张志东[1] 李志勇[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,兰州730046

出　　处：《中国畜牧兽医》2015年第4期823-829,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31201943);国家国际科技合作专项(2011DFA31830);国家高技术研究发展计划(863)项目(2011AA10A211-3)

摘　　要：为筛选与O型口蹄疫病毒(FMDV)衣壳酸敏感性相关的位点,本研究以牛源O型FMDV ON株为研究对象开展耐酸性毒株筛选工作。通过给予pH 6.0酸性环境连续诱变,筛选获得6株耐酸性毒株。将诱变病毒与亲本病毒衣壳蛋白编码区P1区序列比对后发现7个核苷酸突变,即共同存在于6株耐酸毒株P1区的错义突变A1334G(Y445C)、A1643G(Q548R)、A1822G/A1823C(N608A)及同义突变G1371A,另有仅存在于耐酸毒株m2P1区的错义突变C1849T(P617S)和仅存在于耐酸毒株m1P1区的同义突变G468T。本研究为O型FMDV酸敏感性分子机制的揭示及病毒衣壳酸稳定性的提高奠定了基础。The study was aimed to select the acid sensitive sites of foot-and-mouth disease virus （FMDV） type O capsid. Using the bovine FMDV type O ON strain as the research object,six acid resistance monoclonal strains were obtained after treatment in pH 6.0 acidic environment for serial passages. The P1 region which encoding their capsid proteins of the mutagenic viruses was sequenced and compared with the parental virus, then seven mutation sites were found, of which the mutation sites A1334G（Y445C）,A1643G（Q548R）,A1822G/A1823C （N608A） of P1 region found in six strains were sense mutation,while the mutation site G1371A was nonsense mutation. In addition the mutation site C1849T（P617S） of P1 region found in m2 was sense mutation and the mutation site G468T of P1 region found in ml was nonsense mutation. This research laid a good foundation for the reveal of the acid sensitive mechanism and the acid sensitive enhance of FMDV type O capsid.

关 键 词：O型口蹄疫病毒 衣壳 酸敏感性 耐酸位点 

分 类 号：S855.3[农业科学—临床兽医学]