一定纯度中药复方多糖对鸡细胞免疫和体液免疫的影响  

Effects of Certain Purified Chinese Herbal Medicinal Compound Polysaccharide on Cellular Immunity and Humoral Immunity in Chicken

作　　者：商云霞[1] 朱晓庆[1] 谷新利[1] 李效振[1] 乔海博[1] 贾书红[1] 张东升[1] 

机构地区：[1]石河子大学动物科技学院,石河子832000

出　　处：《中国畜牧兽医》2015年第4期943-949,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30960291)

摘　　要：为筛选对鼠免疫增强效果显著的一定纯度中药复方多糖(cCHMPS)对雏鸡的最佳免疫增强剂量,本试验将300只1日龄雄性良凤青脚麻鸡随机分为6组,即空白对照组、盐酸左旋咪唑组、黄芪多糖组及cCHMPS高、中、低剂量组,每组各50只。分别于8日龄皮下注射生理盐水、盐酸左旋咪唑、黄芪多糖及不同剂量的cCHMPS,连续7d,在免疫后第8、14、21、35、42天采血,测定外周血中T、B淋巴细胞增殖活性及血清中新城疫病毒抗体含量。试验结果表明,黄芪多糖和不同剂量cCHMPS均能显著或极显著促进外周血T、B淋巴细胞增殖(P<0.05;P<0.01),提高新城疫病毒抗体浓度,增强机体细胞免疫和体液免疫水平,且中剂量的cCHMPS的免疫增强效果最好。In order to select the best dose of certain purified Chinese herbal medicinal compound polysaccharide （cCHMPS）, which had been proven to significantly affect the immunity of mice, three hundred one-day-old male Liangfeng Green-feet Ma chickens were randomly divided into six groups,namely control group, levamisole hydrochloride （LM） group, Astragalus polysaccharide （APS） group,high,medium and low-dose purified cCHMPS group,and with 50 chickens for each group. Eight-day-old chickens were vaccined with physiologic saline, LM, APS, high, medium and low-dose purified cCHMPS,once a day for 7 consecutive days. On days 8,14,21,28,35 and 42 after the first vaccination,5 chickens＇ blood for each group were sampled for determination of T,B lymphocyte proliferation activities and NDV antibody levels. The results showed that APS and purified cCHMPS at different doses could significantly or extremely significantly promote the proliferation of peripheral T, B lymphocytes （P 〈 0. 05; P 〈 0. 01）, increase the concentrations of NDV antibody, enhance cellular immunity and humoral immunity, and the medium-dose purified cCHMPS was the best.

关 键 词：一定纯度中药复方多糖 黄芪多糖 盐酸左旋咪唑 细胞免疫 体液免疫 

分 类 号：S852.5[农业科学—基础兽医学]