2011-2013年肇庆市禽流感职业暴露人群及外环境病毒分布监测分析  

Surveillance analysis on the high risk population and the distributional environment of avian influenza in Zhaoqing from 2011 to 2013

作　　者：朱碧柳[1] 黄国华[1] 麦炜 谭翰清[1] 黄旭东 陈志鹏[1] 吕灶群 何炜林 周跃华[1] 

机构地区：[1]肇庆市疾病预防控制中心,广东肇庆526020 [2]高要市疾病预防控制中心,广东高要526100

出　　处：《现代预防医学》2015年第8期1386-1388,1391,共4页Modern Preventive Medicine

基　　金：2011年度广东省肇庆市科技创新立项课题(2011E388)

摘　　要：目的了解高致病性禽流感病毒在肇庆市职业暴露人群中的感染状况及其在环境中的分布情况。方法采集禽类职业暴露人员血清标本,用马血球血凝抑制试验(HI)检测H5N1禽流感病毒抗体;采集外环境标本,用实时荧光定量PCR检测A型流感病毒。结果 2011-2013年共采集职业暴露人员血清标本560份,H5N1抗体均为阴性;402份环境标本,阳性89份(阳性率22.14%),H9亚型51份(占57.30%),H5亚型6份(6.74%),H7亚型1份(1.12%),未分型20份(22.47%),混合型11份(12.36%)。活禽市场阳性率远远高于规模养殖场。结论肇庆市职业暴露人群尚未发现感染高致病性禽流感H5N1病毒;环境存在禽流感病毒的污染,H9亚型是主要的病原体;应当加大对活禽市场禽流感病毒监测的力度。Objective The aim of this study was to investigate the infection condition of highly pathogenic H5N1 avian influenza（HPAI） virus in poultry-exposed population and its distribution in the environment in Zhaoqing. Methods The serums were collected from the population and examined by horse haemagglutination inhibition（HI） tests. Real-time fluorescent quantitative PCR methodology was employed to detect the influenza virus from environmental samples. Results It was found that 560 serums were negative from 2011 to 2013. And 89 out of 402 environmental samples were positive with a positive rate 22.14%. The number of positive samples for H9, H5, H7, mixed type and unclassified type were 51, six, one, 11 and 20, respectively. The positive rate in live poultry markets was much higher than that in poultry farms. Conclusion The population was not found to be infected by highly pathogenic avian influenza H5N1 virus. The environment was contaminated with avian influenza A virus. And H9 was the prevailing subtype. Surveillance of bird flu virus on the live poultry markets should be strengthened.

关 键 词：禽流感 职业暴露 监测 

分 类 号：R139.2[医药卫生—劳动卫生]