高效液相色谱法测定空气中的16种多环芳烃  

HPLC determination of 16 different polycyclic aromatic hydrocarbons in air

作　　者：何燕[1] 王淑惠[1] 解彦平[1] 李丽敏[1] 张莹[1] 

机构地区：[1]河北省石家庄市疾病预防控制中心理化所,河北石家庄050011

出　　处：《现代预防医学》2015年第8期1475-1478,1500,共5页Modern Preventive Medicine

摘　　要：目的建立一种前处理简单、灵敏度高、重现性好的空气中16种多环芳烃的检测方法。方法采用高效液相色谱法,Ultimate PAH（250 mm×4.6 mm,5μm）液相色谱柱分离,乙腈-水为流动相进行梯度洗脱,紫外检测器,荧光检测器检测,紫外检测波长230 nm,荧光检测波长248-305 nm。结果测定结果具有良好的线性（R=0.9990-0.9999）,保留时间的RSD为0.007%-0.017%（n=9）,峰面积的RSD为0.08%-2.41%（n=9）,样品加标回收率为63.21%-102.92%,16种多环芳烃同时测定的检出限分别为：萘：0.040μg/ml、芴：0.020μg/ml、苊：0.032μg/ml、菲：0.020μg/ml、蒽：0.024μg/ml、荧蒽：0.020μg/ml、芘：0.032μg/ml、苯并（a）蒽：0.032μg/ml、屈：0.008μg/ml、苯并（b）荧蒽：0.016μg/ml、苯并（k）荧蒽：0.020μg/ml、苯并（a）芘：0.008μg/ml、二苯并（a,h）蒽：0.016μg/ml、苯并（g,h,i）苝：0.032μg/ml、茚苯（1,2,3-cd）芘：0.020μg/ml、苊烯：0.020μg/ml。结论该方法具有操作简便、快速、重复性好、灵敏度高等优点,可用于测定空气中的多环芳烃。Objective The study aimed to establish a method with simple pre-treatment, high sensitivity, and good reproducibility for determination of 16 different polycyclic aromatic hydrocarbons in air. Methods HPLC analysis was performed on an Ultimate PAH column（250mm×4.6mm, 5μm）, with acetonitrile-H2 O as the mobile phase for gradient elution. The samples were detected under 230 nm of UV and 248-305 nm of fluorescence. Results The result showed good linearity（R=0.9990-0.9999）, with a RSD of retention time of 0.007-0.017%（n=9）, a RSD of peak area of 0.08%-2.41%（n=9）, and a recovery rate of 63.21%-102.92%. The detection limits of the 16 different polycyclic aromatic hydrocarbons were 0.040 μg/ml（naphthalene）, 0.020 μg/ml（fluorene）, 0.032μg/ml（acenaphthene）, 0.020 μg/ml（phenanthrene）, 0.024 μg/ml（anthracene）, 0.020 μg/ml（fluoranthene）, 0.032 μg/ml（pyrene）,0.032 μg/ml（benz [a] anthracene）, 0.008 μg/ml（chrysene）, 0.016 μg/ml（benzo [b] fluoranthrene）, 0.020 μg/ml（benzo [k]fluoranthrene）, 0.008 μg/ml（benzo [a] pyrene）, 0.016 μg/ml（dibenz [a,h] anthracene）, 0.032 μg/ml（benzo [g,h,i] perylene）, 0.020μg/ml（indeno [1,2,3-cd] pyrene）, and 0.020 μg/ml（acenaphthylene）. Conclusion The method is simple, fast, repeatable, sensitive,and is thus suitable for determination of polycyclic aromatic hydrocarbons in air.

关 键 词：高效液相色谱 空气 多环芳烃 测定 

分 类 号：R136.3[医药卫生—劳动卫生]