青藤碱对肺癌细胞A549增殖及α7 nAChR表达的影响  

Effect of Sinomenine on Lung Cancer Cell A549 Proliferation and Alpha 7 Nicotinic Acetylcholine Receptor Expression

作　　者：谢冰冰[1] 易浪[1] 董燕[1] 白莎莎[1] 王青[1] 王培训[1] 

机构地区：[1]广州中医药大学免疫研究室,广东广州510405

出　　处：《中药新药与临床药理》2015年第2期164-169,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的研究青藤碱对肺癌细胞增殖的影响及与α7烟碱型乙酰胆碱受体(α7 nAChR)表达的相关性。方法体外培养人肺癌细胞系A549细胞,采用CCK-8法检测24,48h不同浓度青藤碱及4-甲基亚硝氨基-3-吡啶-1-丁酮(NNK)对细胞增殖的影响,流式细胞术检测细胞凋亡情况;RT-PCR法检测A549细胞中α7 nAChR表达。结果0.1,0.25,0.5,1,2,3,4mmol·L-1青藤碱作用于A549细胞后,细胞增殖率随青藤碱剂量增加而降低。NNK能促进细胞增殖,降低细胞早期凋亡比例,并明显增加α7 nAChR的表达;青藤碱可明显抑制NNK的促细胞增殖作用,并提高细胞早期凋亡比例,降低α7 nAChR的表达。结论青藤碱能抑制A549细胞增殖,可能与降低α7 nAChR的表达相关。Objective To observe the effect of sinomenine on proliferation of lung cancer cell A549 and to explore the correlation of its anti-proliferation effect with the expression of alpha 7 nicotinic acetylcholine receptor（α7 nAChR）.Methods Human lung cancer cell line A549 cells were cultured in vitro and were treated by various concentrations of sinomenine and n-nitrosomorpholine（NNK） for 24h and 48h.The effect on cell proliferation activity was assayed by CCK-8 method.The apoptotic rate of A549 cells was detected by flow cytometry,and the expression of α7 nAChR in A549 cells was detected by RT-PCR.Results Sinomenine at 0.1,0.25,0.5,1,2,3,4 mmol·L-1 inhibited the proliferation of A549 cells in a dose-dependent manner.NNK promoted cell proliferation,reduced the proportion of early apoptotic cells and increased the expression of α7nAChR significantly.The effects of NNK were counteracted by sinomenine.Conclusion Sinomenine can inhibit the proliferation of A549 cells,which may be related with the decrease of α7 nAChR expression.

关 键 词：青藤碱 Α7烟碱型乙酰胆碱受体 A549细胞 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]