一种新油茶炭疽病原多基因序列鉴定  

Pathogen identification of a new anthracnose of Camellia oleiferain China based on multiple-gene sequences

作　　者：李河[1] 周国英[1] 徐建平[1] 

机构地区：[1]经济林培育与保护教育部重点实验室,中南林业科技大学林学院,长沙410004

出　　处：《植物保护》2015年第2期92-96,共5页Plant Protection

基　　金：国家自然科学基金(31100479)

摘　　要：从湖南、广西油茶叶上分离获得5株能侵染油茶树的病原菌。通过柯赫氏法则证明该病原菌为油茶炭疽病的致病菌。病菌菌落圆形,初期为白色,逐渐变为浅灰色;气生菌丝由白色和灰白色渐变为深灰色绒状;菌落背面中心颜色深,外部颜色浅;菌丝划伤后在PDA培养基上能产生橘黄色的分生孢子堆,分生孢子为单胞、直、圆柱状、无色、光滑、两头钝圆或一端稍尖,分生孢子大小（11~15）μm×（4~5）μm;菌丝体附着胞不规则状,浅褐色,（8~12）μm×（5.5~7）μm。采用形态学结合多基因（核糖体内转录间隔区（ITS）、钙调蛋白-CAL、3-磷酸甘油醛脱氢酶-GAPDH）系统学的方法对它们进行鉴定,结果发现,5个菌株均为暹罗刺盘孢（Colletotrichum siamense）。这是国内油茶上C.siamense的首次报道。Five strains of anthracnose pathogen of Camellia oleifera were isolated from two provinces of China.The pathogenicity tests were performed using conidial suspension of Colletotrichumsp.on young and healthy leaves of C.oleifera,and the Colletotrichum sp.isolates were confirmed as the pathogenic fungi.Isolations were transferred onto PDA plates and showed circular colonies.These morphological characteristics of colonies were first white and then turned grey and grey black.The pile of spores was jacinth.Conidia were oblong,singlecelled,colorless,（11-15）μm×（4-5）μm in size.Mycelial appressorium was clava-like,brown,smooth-edged and intact,（8-12）μm×（5.5-7）μm in size.We identified these isolates based on morphological characters and phylogenetic analysis of multilocus（ITS,CAL,GAPDH）sequences.The results showed that the pathogen causing anthracnose of C.oleifera was C.siamense.This is the first report of C.siamense on C.oleiferain China.

关 键 词：油茶 COLLETOTRICHUM siamense 炭疽病 病原菌 多基因序列 

分 类 号：S435.659[农业科学—农业昆虫与害虫防治]