不规则抗体筛查结果分析  

Result analysis of irregular antibody screening

作　　者：刘蕾[1] 朱雪[1] 焦晋山[1] 

机构地区：[1]山西医科大学第一临床医学院输血科,太原030001

出　　处：《临床血液学杂志（输血与检验）》2015年第2期300-302,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨人群中不规则抗体的阳性率及与临床安全输血的关系。方法:收集2014-06-01-2014-9-20的1 615例配血者血浆和临床资料做不规则抗体筛查,采用微柱凝胶抗球蛋白法检测红细胞血型不规则抗体,研究不同就诊人群红细胞血型不规则抗体的检出率,对不规则抗体阳性的患者分别做盐水法,微柱凝胶抗球蛋白法和经典抗人球蛋白法,调查不规则抗体的特异性分布及产生规律。结果:1 615例被检者红细胞血型不规则抗体检出15例阳性率0.93%(15/1 615),其中男性检出率为0.67%(4/595),女性检出率为1.08%(11/1 020),女性有妊娠史的患者检出率为1.28%(11/857),无妊娠史患者检出率为0(0/163),有输血史患者290例检出率3.45%(10/290),高于无输血史的患者检出率0.38%(5/1 325),两者比较差异有统计学意义(P<0.01)。抗体特异性以抗RH系统最多见检出6例检出率为40%(6/15),其中,抗-E 4例、抗-D 1例、抗-c 1例;其次是Kell和MNSs系统各4例检出率26.67%(4/15)分别为抗-K 4例和抗-M 1例、抗-s 2例、和抗-Mur 1例;最后还在Duffy系统中发现抗-Fya 1例检出率为6.67%(1/15)。结论:本研究为献血者抗体筛选提供了决策依据,并深入研究在不同就诊人群中不规则抗体的特异性与安全输血的关系。Objective：To discuss positive rate of the irregular antibody screening in the crowd and relationship between the irregular antibody and clinical safe transfusion.Method：Clinical data and sera of 1 615 patients who had received transfusion patients in our hospital from June 1st to September 20 th in 2014 were collected and identified the irregular antibodies by microcolumn gel technique.The irregular antibody detection rate of different visit crowd was studied.Irregular antibody positive was detected by brine method,microcolumn gel antiglobulin method and the classic anti globulin method,respectively,and irregular antibody specificity distribution and production were investigated.Result：Irregular antibody positive of 1 615 cases were 15 cases and detection positive rate was0.93%（15/1 615）.The positive rate of men was 0.67%（4/595）,and the positive rate of women was 1.08%（11/1 020）.The positive rate of female patients with a history of pregnancy was 1.28%（12/857）,positive rate of no pregnancy history group was 0（0/163）.The positive rate of the history of blood transfusion was 3.45%（10/290）,which was much higher than that of no history of blood transfusion 0.38%（5/1 325）,which difference was statistically significant（P〈0.05）.The majority of the antibody specificity checked out was RH system,with 6cases and the detection rate was 40%（6/15）,in which anti-E was 4cases,one of anti-D and one of anti-c.Then Kell and MNSs system respectively was four cases which positive rate was 26.67（4/15）,including four cases of antiK,one cases of anti-M,two cases of anti-s and one cases of anti-Mur.The last,one cases of anti-Fya was found in the system of Duffy,and the positive rate of anti-Fya was 6.67%（1/15）.Conclusion：This study of antibody screening of blood donors would provide a decision-making basis,and further study of relationship between irregular antibody specificity and safety of blood transfusion in the different crowd.

关 键 词：不规则抗体 抗体筛查 安全输血 

分 类 号：R457.1[医药卫生—治疗学]