羊流产嗜性衣原体MOMP基因的克隆及序列分析  

Cloning and sequence analysis of MOMP gene of goat Chlamydophila abortus

作　　者：姚菊霞[1,2] 王光华[1] 陆艳[1] 李秀萍[1] 王戈平[1] 

机构地区：[1]青海省畜牧兽医科学院,西宁810016 [2]甘肃省通渭县动物疫病预防控制中心,通渭743300

出　　处：《青海畜牧兽医杂志》2015年第2期1-4,共4页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：现代农业产业技术体系建设专项经费资助(CARS-40-08B);青海省现代农牧业产业科技创新平台-羊产业科技创新平台

摘　　要：为探讨羊流产嗜性衣原体MOMP蛋白的生物学功能、揭示其在流产衣原体病诊断和防治中的作用。本试验根据Gen Bank公布的流产嗜性衣原体主要外膜蛋白(MOMP)基因序列,设计并合成4条特异性引物,以羊流产嗜性衣原体青海分离株感染的鸡胚卵黄囊中提取的衣原体基因组为模板,应用套式PCR扩增到MOMP全基因序列。通过生物信息学软件对该基因的结构及其推导的氨基酸序列进行了预测分析,同时分析了与其他流产嗜性衣原体的同源性。结果显示,该基因全长1 170 bp,可编码389个氨基酸,蛋白分子量理论值为41.8 ku,理论等电点7.54,编码蛋白理化性质较稳定。其第1位到第19位氨基酸残基为信号肽序列,具有3处N-糖基化位点。含有多个能被其他酶修饰的位点,以无规则卷曲为主,含5个跨膜区,3个亲水性较强的抗原表位。同源性分析表明与其他流产嗜性衣原体分离株的一致性高在93%以上,系统进化分析表明与OCLH196株(序列号为AJ440239)位于同一进化分支。In order to study the function of the major outer membrane protein（MOMP） gene from Goat Chlamydophila abortus, and reveal the role of MOMP in Chamydophilia abortus disease diagnosis and prevention. In this thesis, genomic DNA was extracted from the Chick embryo yolk sac infected with Goat CA. According to the published complete nucleotide sequence of MOMP in Gen Bank,four primers specific to MOMP gene were designed. The MOMP gene fragment was amplified by nested PCR. The nucleotide sequence and the deduced amino acid sequence e were analyzed with the bioinformatics software. The homology of CAQH with other CA MOMP was analyzed. Sequencing analysis results indicated that the c DNA sequence contained an open reading frame（ORF）of 1170 nucleotides. The deduced protein consisted of 389 amino acid residues with the theoretical molecular weight of 41.8 ku and isoelectric point of 7.54. The protein deduced from the gene was stable at physical and chemical characteristics. The signal peptide sequence of MOMP located between amino acids 1 and 19, and 3 N-glycosylation sites were also identified. owning many potential modified sites, dominating by random coil,five over-membrane region and three major linear epitope. The CAQH MOMP nucleotides sequence showed more than 93% identity with other MOMP from CA, phylogenetic analysis indicated that CAQH MOMP located in the same clade with the OCLH196 strain（Accession AJ440239）.

关 键 词：羊流产嗜性衣原体 MOMP基因 克隆 序列 

分 类 号：S852.67[农业科学—基础兽医学]