不可逆电穿孔对兔组织阻抗的影响  

Influence of Irreversible Electroporation on Tissues Resistance of Rabbit

作　　者：董守龙[1] 姚陈果[1] 储贻道 米彦[1] 李成祥[1] 赵亚军[1] 

机构地区：[1]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400030

出　　处：《高电压技术》2015年第4期1402-1408,共7页High Voltage Engineering

基　　金：国家自然科学基金(50637020);重庆市科委自然科学基金(2011jj A70008)~~

摘　　要：为了研究不可逆电穿孔对生物组织阻抗的影响,探究了阻抗变化与不可逆电穿孔机理之间的关系。以新西兰大白兔的多种组织作为对象,对其施加μs级高强度脉冲电场,测量、计算了组织阻抗值的变化。实验采用脉冲参数为:幅值2 250 V,脉冲脉宽70μs,重复频率2 Hz,脉冲总数90个。实验结果表明:生物各组织发生不可逆电穿孔时,其阻抗均减小;随着脉冲作用时间增加,阻抗趋于稳定,但在41个、61个脉冲作用后分别停止5min后阻抗明显恢复,说明可逆电穿孔比例仍较大;随着脉冲电场强度和作用时间的继续增大,不可逆电穿孔数量和程度迅速增大,直至组织阻抗达到最小值;停止施加脉冲电场后,组织阻抗增大,即部分电穿孔自恢复。In order to study how irreversible electroporation influences vivo tissue impedance, we experimentally studied the relationship between impedance change and irreversible electroporation mechanism. Taking the several tissues in New Zealand white rabbits as the research object, we treated them with nanosecond pulsed electric field, and measured the change of vivo tissue impedance. The applied pulsed electric field was with amplitude of 2 250 V, pulse width of 70 μs, and repetitive frequency of 2 Hz, and the total pulse number was 90. Conclusions are drawn as follows. Due to irreversible electroporation, the resistances of all the tested vivo tissues will be decreased. With increasing treatment time, vivo impedance tends to stabilize; but 5-minutes interval after the 41 ^st and 61 ^st pulse brings obvious recovery of vivo impedance, which indicates a relatively large proportion of reversible electroporation in cells. With longer treatment or enhanced electric filed, irreversible electroporation will increase in both quantity and extent, and vivo tissue impedance will reach a minimum value eventually. When the pulsed electric field stops, the vivo tissue impedance will recover, indicating a certain amount of electroporation recovery.

关 键 词：不可逆电穿孔 阻抗 电脉冲 电导率 细胞膜 组织消融 

分 类 号：Q68[生物学—生物物理学]