HPLC法同时测定绿衣枳壳中3种黄酮类化合物的含量  

Simultaneous determination of three flavonoids in Fructus Ponciri Trifoliatae Immaturi by HPLC

作　　者：潘馨[1] 谢秋情 李烜诚 

机构地区：[1]福建中医药大学,福州350122

出　　处：《药物分析杂志》2015年第4期666-669,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：福建省资助省属高校项目(2006F5053);福建省卫生厅课题(wzzy201312);福建省大学生创新项目(20141039035)

摘　　要：目的：建立HPLC法同时测定绿衣枳壳中柚皮苷、橙皮苷及枳属苷的含量。方法：采用Hypersil ODS2（250 mm×4.6mm,5μm）色谱柱,以乙腈（A）-0.1%磷酸水溶液（B）为流动相,梯度洗脱（0~15 min,20%A;15~17 min,20%A→30%A;17~27 min,30%A）,流速1.0 m L·min-1,检测波长283 nm。结果：柚皮苷、橙皮苷及枳属苷进样量分别在0.080~2.667、0.033~1.088、0.090~3.013μg范围内呈良好线性关系（r=0.999 6~0.999 9）;各组分平均回收率（n=3）在99.63%~101.5%之间,RSD为0.040%~1.2%。10批样品测定结果为柚皮苷含量1.62%~2.02%,橙皮苷含量0.045%~0.063%,枳属苷含量1.66%~1.90%。结论：本法可用于同时测定绿衣枳壳中柚皮苷、橙皮苷及枳属苷的含量。Objective： To develop an HPLC method to simultaneously determine naringin,hesperitin and poncirin in Fructus Ponciri Trifoliatae Immaturi. Methods： The Hypersil ODS2（ 250 mm × 4. 6 mm,5 μm） column was used.Acetonitrile（ A）- 0. 1% phosphoric acid（ B） was used as the mobile phase with gradient elution（ 0- 15 min,20%A; 15- 17 min,20% A→30% A; 17- 27 min,30% A）,at a flow rate of 1. 0 m L·min- 1. The detection wavelength was 283 nm. Results： The calibration curves of naringin,hesperitin and poncirin were in good linearity over 0. 080-2. 667 μg,0. 033- 1. 088μg,0. 090- 3. 013μg（ r = 0. 999 6- 0. 999 9）. The average recovery was in the range of 99. 63%- 101. 5% and RSD was in the range of 0. 040%- 1. 2%. The contents in 10 samples were in the range of1. 62%- 2. 02% for naringin,0. 045%- 0. 063% for hesperidin and 1. 66%- 1. 90% for poncirin. Conclusion：The method can be used to determine the contents of naringin,hesperitin and poncirin in Fructus Ponciri Trifoliatae Immaturi.

关 键 词：绿衣枳壳 柚皮苷 橙皮苷 枳属苷 中药质量控制 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]