HPLC法测定芪胶升白胶囊中朝藿定C和淫羊藿苷的含量  

Determination of epimedin C and icariin components in Qijiaoshengbai capsules by HPLC

作　　者：龙国友 李开斌 刘兴鹏[1,2] 程显隆[3] 魏锋[3] 马双成[3] 熊慧林[2] 茅向军[2] 

机构地区：[1]贵州省安顺市食品药品检验所,安顺561000 [2]贵州省食品药品检验所,贵阳550000 [3]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2015年第4期719-722,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家药品标准提高研究课题

摘　　要：目的：建立中成药芪胶升白胶囊中朝藿定C和淫羊藿苷的含量测定方法。方法：采用高效液相色谱法,使用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水（24.5∶75.5）为流动相,流速1 m L·min^-1,检测波长270 nm,柱温25℃。结果：朝藿定C进样量在0.027~0.542μg范围内线性良好（r=0.999 8）,淫羊藿苷进样量在0.025~0.499μg范围内线性良好（r=0.999 9）。朝藿定C的平均回收率（n=6）为99.27%,RSD为1.2%;淫羊藿苷的平均回收率（n=6）为100.2%,RSD为1.6%。测得3批次芪胶升白胶囊中的朝藿定C、淫羊藿苷的平均含量范围为0.570~0.599 mg·g-1、0.395~0.409 mg·g-1。结论：本方法经方法学验证,可作为芪胶升白胶囊中朝藿定C和淫羊藿苷含量测定的方法。Objective： To establish a method for determination of epimedin C and icariin in Chinese patent medicine Qijiaoshengbai capsules. Methods： HPLC was used with the Diamonsil C18（ 250 mm × 4. 6 mm,5μm） column,acetonitrile- water（ 24. 5∶ 75. 5） as the mobile phase at a flow rate of 1. 0 m L·min^- 1,the detecting wavelength of 270 nm,and the column temperature at 25 ℃. Results： The good linear was over the range of 0. 027- 0. 542 μg for epimedin C（ r = 0. 999 8） and the average recovery（ n = 6） was 99. 27% with RSD of 1. 2%; the good linear was over the range of 0. 025- 0. 499 μg for icariin（ r = 0. 999 9） and the average recovery（ n = 6） was 100. 2% with RSD of 1. 6%. The content ranges in three batches of Qijiaoshengbai capsules were 0. 570- 0. 599 mg·g- 1for epimedin C and 0. 395- 0. 409 mg·g- 1 for icariin. Conclusion： This validated method can be used for the determination of epimedin C and icariin in Qijiaoshengbai capsules.

关 键 词：芪胶升白胶囊 淫羊藿 箭叶淫羊藿 柔毛淫羊藿 朝鲜淫羊藿 朝藿定C 淫羊藿苷 高效液相色谱 方法验证 

分 类 号：R917[医药卫生—药物分析学]