基于原子吸收法探究血液灌流对血铊的清除作用  

Efficiency of hemoperfusion on clearing thallium based on atomic absorption spectrometry

作　　者：田甜[1] 王永安[2] 聂志勇[2] 王姣[1] 彭晓波[1] 原野[2] 李万华[2] 邱泽武[1] 薛艳萍[3] 熊艺茹[3] 

机构地区：[1]100071北京,全军中毒救治中心,军事医学科学院附属医院中毒救治科 [2]100850北京,抗毒药物与毒理学国家重点实验室,军事医学科学院毒物药物研究所 [3]100020北京,首都医科大学附属北京朝阳医院放射科,医务部科训科

出　　处：《中华危重病急救医学》2015年第4期259-262,共4页Chinese Critical Care Medicine

基　　金：国家新药创制重大专项资助（2011ZXJ09102-05C,2011ZXJ-09305,2011ZXJ09102）;全军医学科技“十二五”重大（AWSlIC004）及重点（BWSl2J042）项目

摘　　要：目的：基于原子吸收法检测犬全血铊含量，观察血液灌流（HP）对血中铊的清除效能。方法经股动脉取未染毒比格犬的血液，根据实验动物体质量与血容量的换算公式，配制成3组浓度梯度的含硝酸亚铊（TlNO3）的犬血，充分模拟血液在体环境进行HP，以前一剂量组最后一次灌流结束后犬血中所含TlNO3的总量确定下一组含铊血液样品的配制。应用石墨炉原子吸收仪检测每次灌流前后犬全血铊含量，并对其进行统计分析。结果犬全血铊含量在0～200μg/L线性良好，相关系数为0.9984；日内精密度＜4.913%，日内回收率96.2%～111.9%；日间精密度＜7.502%，日间回收率89.6%～105.2%。高、中、低剂量组每次灌流后血铊浓度均明显降低〔高剂量组从（453.43±27.80）mg/L降至（56.09±14.44）mg/L，F＝8.820，P＝0.003；中剂量组从（64.51±13.60）mg/L降至（3.19±0.23）mg/L，F＝36.312，P＝0.000；低剂量组从（5.40±0.98）mg/L降至（0.38±0.25）mg/L，F＝46.240，P＝0.000〕。高、中、低剂量组4次灌流后总体吸附率分别为（87.63±2.48）%、（95.06±1.54）%、（92.76±4.87）%，组间比较差异无统计学意义（F＝4.231，P＝0.070）。结论采用石墨炉原子吸收仪检测血铊浓度定量准确、灵敏度高、稳定性强，所测样品处理简单，保存时间长，适合批量样本的检测。HP可以稳定、高效地清除血中的铊，4次灌流即可吸附90%以上的铊；且HP的吸附效率与血铊含量无关，即使血铊含量低，HP也是有效的。ObjectiveTo determine thallium in whole blood by atomic absorption detection method, and to investigate the eliminating effect of hemoperfusion （HP） for thallium in blood.Methods The blood of Beagle dogs which had not exposed to thallium before were obtained for preparation of thallium nitrate （TlNO3）-containing solution in three concentrations according to the conversion formula based on animal weight and volume of blood. HP was performed in the simulated in vivo environment. The content of TlNO3 in blood of the next group was determined on the amount of TlNO3 for the last HP of the former dose group. Thallium quantity in different samples was measured with atomic absorption spectrometer blood samples before and after HP. Finally, the thallium concentration in blood was analyzed statistically.Results Thallium concentrations showed a good linear relationship in the range of 0-200μg/L （r = 0.998 4）. The intra-day precision （RSD） was lower than 4.913%, the intra-day recovery rate was 96.2%-111.9%; the inter-day precision （RSD） was lower than 7.502%, the inter-day recovery rate was 89.6%-105.2%. The concentration of thallium in blood was significantly reduced after HP per time in high, middle, and low dose groups [（453.43±27.80） mg/L to （56.09±14.44） mg/L in high dose group,F = 8.820,P = 0.003;（64.51±13.60） mg/L to （3.19±0.23） mg/L in middle dose group,F = 36.312,P = 0.000; （5.40±0.98） mg/L to （0.38±0.25） mg/L in low dose group,F = 46.240,P = 0.000]. The adsorption rate of four times of HP in high, middle and low dose group were （87.63±2.48）%, （95.06±1.54）% and （92.76±4.87）%, respectively, without significant＆amp;nbsp;difference （F = 4.231,P = 0.070 ）.Conclusions The method for measuring thallium was established, and it shows a very stable, simple, sensitive for determination of thallium. HP can effectively remove thallium from blood. Thallium concentration can be reduced by 90% after four times of HP. HP is also effective even when thallium con

关 键 词：铊 中毒 血液灌流 炭肾 

分 类 号：O657.31[理学—分析化学] R927.2[理学—化学]