黄芪对急性肺损伤模型肺组织中细胞间粘附分子-1和血管细胞间粘附分子-1表达的调节作用  

Effects of Astragalus on Intercellular Adhesion Molecule- 1 and Vascular Cell Adhesion Molecule- 1 in Rats of Acute Lung Injury

作　　者：周易[1,2] 李火平[1,2] 夏光明[1,2] 张慧[1,2] 金朝霞[1,2] 周帆[1,2] 石素平 

机构地区：[1]黄冈市中心医院 [2]长江大学附属黄冈医院医学实验中心,湖北黄冈438000

出　　处：《中国药物警戒》2015年第4期197-199,共3页Chinese Journal of Pharmacovigilance

摘　　要：目的观察黄芪对急性肺损伤的保护作用并探讨肺组织中细胞间粘附分子-1(ICAM-1)、血管细胞间粘附分子-1(VCAM-1)的表达在急性肺损伤(ALI)中的作用。方法 Wistar大鼠30只,随机分为正常对照组、模型组和黄芪组,每组10只。建立急性肺损伤模型,给药6 h后处死所有大鼠,取出肺组织做病理学观察,并检测各组肺组织髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性变化以及ICAM-1、VCAM-1的表达变化。结果与正常对照组相比,模型组、黄芪组的MPO活性和ICAM-1、VCAM-1表达均显著增强(P<0.05或P<0.01),且黄芪组上述指标明显低于模型组(P<0.05或P<0.01);急性肺损伤后,肺组织中的SOD活性明显降低(P<0.05或P<0.01),但经黄芪处理后的黄芪组SOD活性水平又明显的增强(P<0.05)。结论黄芪能对急性肺损伤能起到有效的保护作用,其机制可能与其调节ICAM-1、VCAM-1表达和提高SOD活性有关。Objective To investigate the protective effects of Astragalus on acute lung injury and to explore the role of intercellular adhesion molecule-1 （ICAM-1） and vascular cell adhesion molecule-1 （VCAM-1） on acute lung injury in rats. Methods 30 Wistar rats were randomly divided into control group, model group and Astragalus group （n=10 , respectively）. All rats were sacrificed 6 h after administration of Astragalus, removed lung tissue for pathological observation, and examined the expression of myeloperoxidase （MPO）, superoxide dismutase （SOD） activity and ICAM-1, VCAM-1 in each group. Results Compared with control group, MPO activity and ICAM-1, VCAM-1 expression were significantly increased in model group and Astragalus group （P 〈0.05 or P 〈0.01 respectively）.However, in Astragalus group, MPO activity and ICAM-1, VCAM-1 expression were significantly lower than that of model group （P 〈0.05 or P 〈0.01 respectively）. Lung tissue SOD activity was significantly lower after lung injury （P 〈0.05 or P 〈0.01 respectively）, but it was significantly enhanced after treatment with Astragalus （P 〈0.05）. Conclusion Astragalus can play a protective effect on ALI, and the mechanism may relate to regulate the expression of ICAM-1, VCAM-1 and increase the activity of SOD.

关 键 词：黄芪 急性肺损伤 髓过氧化物酶 超氧化物歧化酶 粘附分子 

分 类 号：R965[医药卫生—药理学]