柴胡水提组分对大鼠解热及伴随毒副作用实验研究  

Study on Rat's Antipyretic Effect Accompanied by Side Effects of Water Extraction Components of Bupleurum

作　　者：石亮[1,2] 黄娜娜[2] 孙晓倩[2] 李晓宇[1] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2015年第4期203-206,共4页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金面上项目(81374059);山东省自主创新和成果转化项目(2014ZZCX02104);山东省自然基金重点项目(2011ZRB14333)

摘　　要：目的研究柴胡水提组分对大鼠发热模型的解热作用及伴随的毒副作用,为其"功效-毒性"相关性研究提供实验依据。方法采用背部皮下注射2,4-二硝基苯酚,制备大鼠发热模型,灌胃不同剂量柴胡水提组分,每30 min测体温1次,连续测定4 h,记录肛温,观察其解热作用;检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆汁酸(TBA)、总胆红素(TBIL)、肌酐(Cr)、尿素氮(BUN)的活性及含量,剖取心脏、肝脏、肾脏称重,计算心、肝、肾各脏体比值,评价其药效伴随毒副作用。结果柴胡水提组分对2,4-二硝基苯酚所致大鼠发热模型有降温作用,给药3 d后血清ALT、AST活性明显升高,肝体比值无明显变化。结论药效剂量的柴胡水提组分对大鼠发热模型有一定的降温作用,同时会产生一定的肝毒性,并呈现一定的剂量依赖关系。Objective To study the antipyretic effects and accompanied side effects of water extraction of Bupleurum components for rat fever model so as to provide the experimental basis for ＆quot;efficacy- toxicity＆quot; correlation. Methods Rat fever model was established via the back injection of 2,4-dinitrophenol （DNP）. Different doses of water extraction of Bupleurum components were given to rats by gavage. The temperature was detected in anus once every 30 minutes, continuously determined for 4 hours, recorded anal temperature and observed the antipyretic effect. At the same time, the levels of ALT, AST, TBA, TBIL, Cr and BUN in serum were detected and the heart, liver and kidney indexes were calculated. Efficacy and accompanied side effects were evaluated via all these data. Results Antipyretic effect was significantly reflected when rat fever model caused by DNP were treated with water extraction of Bupleurum components by gavage. The activities of ALT and AST increased obviously after administration for consecutive 3 days. Conclusion Water extraction of Bupleurum components has the effect of dropping in temperature to the rat fever model, which is accompanied by hepatotoxicity. The effect and hepatotoxicity all show a certain dose dependence relationships.

关 键 词：柴胡 解热 伴随毒副作用 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]