羊口疮病毒B2L基因克隆及表达  

Cloning and Expression of ORFV B2L Gene

作　　者：赵文博[1] 李瑞航[1] 贺鹏亮 李朋娅 王梦醒[1] 杨春华[1] 于永忠[1] 崔玉东[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2015年第2期42-45,共4页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金面上项目(31172353)

摘　　要：为了获得羊口疮病毒囊膜蛋白。以羊口疮病毒基因组为模板,利用羊口疮病毒B2L基因特异性引物,进行PCR扩增,回收以及纯化PCR产物,连接到PMD18-T载体上,测序结果表明B2L基因全长1 137 bp,编码379个氨基酸。再将B2L基因克隆至p ET-30a,构建原核表达载体p ET30a-B2L。经菌液PCR、酶切鉴定以及SDS-PAGE等方法表明成功构建原核表达载体,这将为今后的单抗制备以及羊口疮病毒的疫苗研究打下基础。To obtain ORFV envelope protein and take ORFV genome as template,ORFV B2 L gene was used as specific primers to amplify PCR and recovery and purify PCR products. Connected PCR products to PMD18-T vector,the sequence result showed that full-length of B2 L gene was 1137 bp,and Encoding was 379 amino acids. B2 L gene was cloned into p ET-30 a to construct prokaryotic expression vector p ET30a-B2 L. Prokaryotic expression vector was constructed by the methods of bacterium liquid PCR,enzyme identification and SDS-PAGE,which would provide the research basis of monoclonal antibody and ORFV vaccine in the future.

关 键 词：羊口疮病毒 基因克隆 原核表达 

分 类 号：TQ645.9[化学工程—精细化工]