慢性乙肝患者血清HBV-DNA及前S1抗原检测结果分析  

Analysis of results in detection of HBV-DNA in serum of patients with chronic hepatitis Band Pre-S1 antigen in 85 cases

作　　者：王启龙[1] 

机构地区：[1]邳州市人民医院检验科,江苏邳州221300

出　　处：《中国处方药》2015年第4期5-6,共2页Journal of China Prescription Drug

摘　　要：目的对乙肝患者血清HBV-DNA与前S1抗原检测的方法进行探讨,了解检测HBV-DNA及前S1抗原在诊断乙肝中的作用。方法选取85例乙型肝炎患者,抽取患者的肘静脉血清,采用荧光定量聚合酶链反应(FQ-PCR)进行HBV-DNA检测,使用酶联免疫吸附法(ELISA)对患者的乙肝"两对半"、前S1抗原进行检测,并对两种方法的检测结果进行统计学分析。结果乙肝患者经过检测后,在不同乙肝"两对半"的模式下,乙肝患者的HBV-DNA以及前S1抗原的阳性率与HBV-DNA的阳性率差异并无统计学意义(P>0.05)。结论在对乙肝患者进行检测时,两种方法检测的结果具有一致性、有效性,必要时采用两种方法进行联合检测,能够为乙肝患者的临床疗效考核与预后治疗提供必要的实验室数据。Objective To understand the effect of detecting HBV-DNA and Pre-S1 antigen in the diagnosis of hepatitis B patients through probing into the detection methods of the HBV-DNA and Pre-S1 antigen of hepatitis B patients＇ serum. Methods Selected 85 cases of hepatitis B patients, extract cubital vein serum of the patients, then detected HBV-DNA with fluorescence quantitative polymerase chain reaction（FQ-PCR）, while detected ＂two pairs of semihepatitis B＂ and Pre-S1 antigen with enzyme-linked immunosorbent assay（ELISA）, and the detection results of two methods were statistically analyzed. Results After detection, the positive rate difference between HBV-DNA along with Pre-S1 antigen and HBV-DNA of hepatitis B patients was not statistically significant（P〉0.05） in different modes of ＂two pairs of semi-hepatitis B＂. Conclusion In detecting hepatitis B patients, the results of the two methods are consistent and effective. Two methods should be combined to make the detection when necessary, thus providing necessary laboratory data for clinical efficacy assessment and prognosis treatment of the hepatitis B patients.

关 键 词：乙肝 前S1抗原 HBV-DNA 

分 类 号：R512.62[医药卫生—内科学]