山西道地药材连翘组织培养快速繁育技术研究  

Study on the Rapid Propagation Technique for Forsythia suspensa( Thunb.) Vahl by Tissue Culture of Shanxi Trueborn Medicinal Material

作　　者：吴潇[1] 刘阳[1] 张咪咪[1] 师慧丽[1] 杨忠义[1] 

机构地区：[1]山西农业大学园艺学院,山西晋中030801

出　　处：《安徽农业科学》2015年第12期41-42,47,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划项目(2006BAD05B10);山西省科技基础条件平台建设项目(2010091016);山西农业大学引进人才博士科研启动项目(XB2010017);山西省科技重大专项(20121101010);山西省普通高校大学生创新训练及山西农业大学大学生科技创新项目(11-091)

摘　　要：[目的]试验选取山西道地药材连翘,利用组织培养技术进行快速繁育研究。[方法]以连翘含有2个腋芽的嫩茎茎段为外植体,利用不同激素种类和浓度水平通过正交试验法进行比较,最终利用方差分析筛选出最适宜连翘快速繁育的组织培养体系。[结果]连翘的最佳组织培养体系为初代培养基(MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+蔗糖25 g/L)、增殖培养基(MS+6-BA 1.0 mg/L+IBA 0.1mg/L+蔗糖35 g/L)、生根培养基(MS+6-BA 1.5 mg/L+IBA 0.1 mg/L+蔗糖25 g/L),生根率可达93.3%,生根的平均根长最长,达9.7mm,适合大规模繁殖。[结论]该研究为连翘的大规模发展提供技术支持。[ Objective ] The research aimed to select Shanxi trueborn medicinal material to study the rapid propagation technique for Forsythia suspensa（ Thunb. ）Vahl by tissue culture. [ Method ] Stems with two axillary buds, Forsythia suspensa （Thunb.）Vahl was used as explants for comparison by means of the orthogonal test in different hormone types and concentrations. The optimal tissue culture system of rapid propagation of Forsythia suspensa（Thunb. ）Vahl by variance analysis were established as follow. [ Result ] The optimal tissue culture system of rapid propa- gation of Forsythia suspensa（Thunb. ）Vahl were initial medium （ MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.3 mg/L ＋ surcourse 25 g/L）, enrichment medium（ MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.1 mg/L ＋ surcourse 35 g/L） ,rooting medium（ MS ＋ 6-BA 1.5 mg/L ＋ IBA 0.1 mg/L ＋ surcourse 25 g/L）. The rooting rate was high as 93.3%. The average root length was long as 9.7 mm, which was the longest one. It was suitable for large-scale propagation. [ Conclusion] The study provides the technical support for large - scale development of Forsythia suspensa （Thunb.） Vahl.

关 键 词：连翘 正交试验 组织培养 快速繁育 方差分析 

分 类 号：S567[农业科学—中草药栽培]