北京地区Rh(D)阴性献血者分型及不规则抗体筛查  

Investigation of Rh phenotype detecting and serological irregular antibody screening in the D antigen negative blood donors in Beijing area

作　　者：车进 杨亚玲[2] 李美霖 

机构地区：[1]北京市通州区中心血站质控科,101100 [2]中国人民解放军第306医院输血科

出　　处：《山西医药杂志》2015年第7期740-742,共3页Shanxi Medical Journal

基　　金：首都卫生发展科研项目(首发2014-4-7086)

摘　　要：目的研究北京地区Rh(D)阴性献血者Rh血型系统抗原分型及各型在ABO血型系统中的分布规律并进行不规则抗体的筛查、特异性鉴定。方法 U型板微量盐水法筛选Rh(D)阴性献血者、间接抗人球蛋白法试验和吸收放散试验进行确认,单克隆抗体血清试剂检测献血者红细胞Rh血型系统的C、c、E、e抗原,盐水法和间接抗人球蛋白试验筛查不规则抗体。结果 249份初筛为RhD阴性的献血者确认结果为弱D/部分D19例(7.6%)、DEL 26例(10.4%)、阴性204例(81.9%),筛查出不规则抗体3例(1.2%);204份阴性献血者中各血清学表型以ccee(63.7%)和Ccee(24.5%)为主。结论对本地区Rh(D)阴性献血者血清学分型及不规则抗体进行调查,对建立Rh(D)阴性献血者资料库,提高输血安全有重要意义。Objective To investigate the Rh phenotype in the RhD negative blood donors＇ as well as its dis- tributing regularity and to screen the irregular antibody and identify its specificity. Methods First, we screen the RhD blood donors with the method of microplate saline. Then, we use indirect antiglobulin test and the absorption- elution testing to confirm it. We use monoclonal antibody serum reagent to detect the antigen of C, c, E and e of the blood donors＂ erythrocyte and screen the irregular antibody by saline method and indirect antiglobulin method. Results Nineteen samples （7.6%） were weak D or partial D, 26 samples （10.4%） were DEL and 204 samples （81.9 %） were confirmed RhD negative among 249 RhD negative, which were preliminary screened. Three irregu- lar antibodies （1.2%）were screened in 249 samples. The distribution of RhD negative phenotypes in Beijing area was ccee （63.7 %） and Ccee（24.5 %）. Conclusion It would be valuable in assurance of transfusion safety that in- vestigating the phenotype of Rh and screening the irregular antibody to establish the data bank of blood donors of RhD negative.

关 键 词：RH-HR血型系统 血液鉴定和交叉配血 不规则抗体筛查 

分 类 号：R457.1[医药卫生—治疗学]