合成气发酵梭菌C.autoethanogenum的生长特性与CO发酵性能  

Growth Characteristics and Carbon Monoxide Fermentation Performance of Clostridium autoethanogenum

作　　者：徐惠娟[1,2] 梁翠谊[1] 许敬亮[1] 郭颖[1] 张宇[1] 庄新姝[1] 袁振宏[1] 

机构地区：[1]中国科学院广州能源研究所∥可再生能源重点实验室,广东广州510640 [2]中国科学院大学,北京100049

出　　处：《华南理工大学学报（自然科学版）》2014年第11期136-142,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家"863"高技术计划项目(2013AA065803);广州市科技计划项目(2014J4100220);生物反应器工程国家重点实验室开放课题(2014OPEN10);中国科学院广州能源研究所所长创新基金重点培育项目(y407p11001)

摘　　要：为探讨合成气发酵乙醇的新工艺,对梭菌C.autoethanogenum的生长特性及CO发酵性能进行了研究.考察了不同碳源对C.autoethanogenum生长的影响,发现木糖是其生长的最佳底物.对生长培养基进行了改良,使C.autoethanogenum的菌体质量浓度提高2倍以上,代谢产物以乙酸为主,只产生少量乙醇.利用1 L气体采样袋研究了C.autoethanogenum的CO发酵性能,在1.0 g/L酵母膏的发酵培养基中经过两次CO发酵后,乙醇质量浓度达3.464g/L,CO乙醇转化率达51.7%.研究表明:N2环境、180℃、4MPa下,20min高温液态水处理蔗渣得到的水解液可用于C.autoethanogenum的培养,培养液再进行CO发酵,经过一次发酵后得到的乙醇质量浓度为3.13g/L,CO乙醇转化率为46.9%,与之前两次发酵的结果接近,但是发酵时间大大缩短.In order to develop the new technology of ethanol production from synthesis gas fermentation,the growth characteristics and carbon monoxide（ CO） fermentation performance of C. autoethanogenum were investigated. The effects of different carbon sources on the growth of C. autoethanogenum were analyzed and the results show that xylose is the optimal substrate. After the growth medium being modified,the cell density of C. autoethanogenum increased by more than 2 times,and the metabolic products were mostly acetic acid with a small amount of ethanol.Moreover,the CO fermentation performance of C. autoethanogenum was studied in 1L gas sampling bags. After twice CO fermentation with C. autoethanogenum in the fermentation medium containing 1. 0 g / L yeast extract,an ethanol concentration of 3. 464 g / L was achieved,and the yield of ethanol from CO reached 51. 7%. The investigation results also indicate that the hydrolysate of bagasse treated with hot liquid water at 180 ℃ in a 4 MPa N2 atmosphere for 20 min can be utilized to culture C. autoethanogenum,and the corresponding ethanol concentration and ethanol yield from CO can reach 3. 13 g / L and 46. 9%,respectively after only once CO fermentation,which are similar to the results of the twice CO fermentation but with much less culture time.

关 键 词：合成气 乙醇 一氧化碳 气体发酵 

分 类 号：Q815[生物学—生物工程]