甜荞FaeseuFUL基因的克隆和序列结构分析  

Cloning and Sequence Analysis of FaeseuFULfromFagopyrum esculentum

作　　者：齐蕊[1] 刘志雄[1] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025

出　　处：《长江大学学报（自科版）（下旬）》2015年第1期52-56,5-6,共5页Journal of Yangtze University

基　　金：国家自然基金科学项目(31101202);湖北省教育厅重点科研基金项目(D20101306);湖北省人事厅科研项目(鄂人社函【2011】762号)

摘　　要：利用基因同源克隆技术结合3’RACE和5’RACE基因克隆技术,从甜荞(Fagopyrum esculentum)花芽中克隆到1个与花序分生组织发育和果实发育等过程中起到重要作用的euFUL基因的同源基因FaeseuFUL(GenBank登录号为KM386626)。序列分析表明,其cDNA全长1060bp,包括38bp的5’-UTR、1个编码231个氨基酸的696bp的完整开放阅读框以及326bp的3’-UTR。蛋白质分子序列比对和分子系统进化分析表明,FaeseuFUL基因是MIKC-type MADS-box基因,属于AP1/SQUA亚家族的euFUL进化系,与拟南芥的AGL8/FUL的同源基因相似性达到81.4%,其编码区的C末端含有euFUL进化系的FUL基序和paleoAP1基序。The cDNAs of one FUL homologous gene,FaeseuFUL,were cloned using homology cloning and 3'RACE,as well as 5'RACE method based on the sequence database of NCBI.The fulllength of Faeseu FUL cDNA was 1060 bp,containing an opening frame(ORF)of 696 bp which encode a polypeptide of 231 amino acids,as well as a 38 bp 5'untranslated region(5'-UTR)and a 326 bp 3'-UTR including apoly-A tail.Sequence comparison and structure analysis showed that Faeseu FUL is a gene of family of MIKC-type MADS-box,highly homologous with protein FUL in Arabi dopsisand belonged to the clade of eu FUL which is a clade of subfamily of AP1/SQUA.In the CTerminal region of Faeseu FUL,there is a FUL motif and paleoAP1 motif.

关 键 词：甜荞(Fagopyrum esculentum) FaeseuFUL euFUL MIKC 

分 类 号：Q78[生物学—分子生物学]