罗田迟熟板栗品种SSR分子标记初步鉴定  

Preliminary Study on the Genetic Resources of Luotian Chestnut by SSR Markers

作　　者：李琳玲[1,2] 程华[1,2] 陈小玲[1,2] 余海洋[1,2] 程水源[3] 

机构地区：[1]经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000 [2]黄冈师范学院生命科学学院,湖北黄冈438000 [3]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《长江大学学报（自科版）（下旬）》2015年第3期52-56,6,共5页Journal of Yangtze University

基　　金：湖北省自然科学基金重点资助项目(2010CBB03901);湖北省教育厅高校产学研合作重点资助项目(C2010060)

摘　　要：以湖北省罗田县8个迟熟板栗主要栽培品种为材料,利用SSR(Simple Sequence Repeat)分子标记对8个板栗品种进行初步认证及亲缘关系分析。结果表明:从8条SSR引物中筛选出4条能够扩增出清晰、稳定条带的引物;4条引物共扩增出36条带,其中多态性条带有31条,多态性条带比率为81.6%。UPGMA法聚类分析结果表明,8个板栗品种大致被分为2类;羊毛栗单独一类;其余7种一类,其中油栗、油栗东亲缘关系较近,桂花香1号、桂花香2号、桂花香3号亲缘关系较近,九月寒1号、九月寒3号亲缘关系较近。初步鉴定结果可为板栗品种鉴别、分类及种质资源的有效利用提供依据。8chestnut varieties from Hubei Luotian County as materials,the preliminary analysis of authentication and the genetic relationship of 8chestnut can be varieties using SSR markers were studied.The results showed that:4of the SSR primers screened from the SSR primers bank can amplify clear and stable bands;a total of 4primers amplified 36 bands,the polymorphic with 31,the percentage of polymorphic loci was81.6%.The UPGMA cluster analysis results indicated that 8varieties of Chinese chestnut can be roughly divided into 2classes.Yangmao chestnut is a separate category;the remaining 7in another category,in which You chestnut,Youdong chestnut are of near genetic relationship,the Guihuaxiang of No.1,No.2,No.3,are of near genetic incense relationship,Jiuyuehan No.1and No.3are the closer genetic relationship.Preliminary identification results for chestnut variety identification can provide a basis for the effective use of classification and germplasm resources.

关 键 词：迟熟板栗 SSR标记 引物分离筛选 遗传距离 

分 类 号：Q78[生物学—分子生物学] S664.2[农业科学—果树学]