重楼皂苷对肝纤维化大鼠纤维化标志物的影响及其相关性分析  

Effect of Chonglou saponin on markers of liver fibrosis of hepatic fibrosis rats and correlation analysis

作　　者：洪燕[1] 韩燕全[2] 罗欢[2] 桂洁[2] 张凯[1] 姜辉[2] 

机构地区：[1]安徽中医药大学,安徽合肥230031 [2]安徽中医药大学第一附属医院 国家中医药管理局中药制剂三级实验室,安徽合肥230031

出　　处：《山西中医学院学报》2014年第6期20-22,67,共4页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家中医药重点学科临床中药学建设项目{国中医药人教发[2012]32号};安徽中医药大学自然科学基金项目(2013zr010)

摘　　要：目的:探讨重楼皂苷对四氯化碳(CCl4)诱导的肝纤维化大鼠肝功能以及肝纤维化标志物的影响及其相关性。方法:SD大鼠随机分为正常组、模型组,重楼皂苷低、中、高剂量组,复方鳖甲软肝片组、秋水仙碱组。除正常组外,其余各组采用CCl4致肝纤维化造模。第7周后,正常组灌胃生理盐水,其余各组灌胃相应药物,1次/d。末次给药后,禁食8 h,处死动物取血,ELISA法检测大鼠血清ALT、AST、肝纤四项指标(CⅣ、PCⅢ、LN、HA)含量以及肝组织HYP水平。结果:与模型组比较,重楼皂苷各剂量组大鼠血清ALT、AST和肝纤四项指标含量、肝组织HYP含量降低(P<0.05,P<0.01)。ALT、AST含量与CⅣ、PCⅢ、LN、HA含量具有相关性(P<0.05,P<0.01)。结论:重楼皂苷具有一定的保肝降酶作用,能减轻肝纤维化程度。Objective：To study the protective effect of Chonglou saponin on liver function and hepatic fibrosis markers of hepatic fibrosis rats induced by carbon tetrachloride. Method：SD rats were randomly divided into normal group,model group,Fufang Biejia Ruangan tablets group,colchicine group,Chonglou saponin groups of high,medium and low dosage.After administration,the levels of ALT,AST,HA,LN,C Ⅳ,PC Ⅲ in serum and HYP in liver tissure were measured by ELISA assay. Results：Compared with model group,the levels of ALT,AST,liver fibrosis four indicators in serum and HYP in liver tissue of Chonglou saponin groups were reduced（P〈0.05,P〈0.01）. The contents of ALT,AST and HYP were associated with the contents of HA,LN,CⅣ,PCⅢ（P〈0.05,P〈0.01）. Conclusion：Chonglou saponin has certain effect of protecting liver and reducing enzyme,and could reduce the degree of liver fibrosis.

关 键 词：重楼皂苷 肝纤维化 相关性分析 

分 类 号：R285[医药卫生—中药学]