考马斯亮蓝法检测活性污泥中蛋白质含量优化  

Optimization of Determination of Protein in Activated Sludge by Bradford Method

作　　者：陈茂霞[1,2] 周后珍[1,2] 朱晓华[1,2] 谭周亮[1,2] 李旭东[1,2] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]中国科学院环境与应用微生物重点实验室,四川成都610041

出　　处：《环境科学与技术》2015年第4期1-5,共5页Environmental Science & Technology

基　　金：科技部国际科技合作与交流专项(2013DFG92600);国家自然科学基金(51208489);中国科学院环境与应用微生物重点实验室开放基金项目(KLCAS-2012-02)

摘　　要：采用考马斯亮蓝法对活性污泥中蛋白质的含量进行测定,并从污泥洗涤、蛋白质提取到蛋白质检测,对该方法用于活性污泥蛋白质含量检测进行优化。试验结果表明：采用Tris-HCl缓冲液洗涤、碱提取法法提取、5~10 min水浴、中性或弱酸、弱碱条件下检测,蛋白质浓度在10~180μg/m L内与吸光度具有良好的线性关系（R2=0.995 7）;该方法的精密度（相对标准偏差（RSD）=3.80%）较高,重复性较好（RSD=2.09%）,加样回收率（n=6）范围为96.99%~109.14%。该方法通过优化后具有快速、精确、可靠的优点,可用于活性污泥蛋白质含量的检测。The protein content in activated sludge was detected by Bradford Method which was optimized through the procedure that from washing of sludge, extraction of protein to detection of protein content. The results showed great linear relationship in range of 10-180 μg/mL of protein （R2=0.995 7） with the operation conditions of washing by Tris-HC1 buffer solution, extraction by alkaline, water-bath for 5 ~10 min and detection under neutral, weak-acid or weak-alkaline conditions. The optimized method showed high precision with RSD 3.80%, good reappearance with RSD 2.09% and the average recovery of the additional sample （n=6） was 96.99%~109.14%. This method which is rapid, accurate and reliable, could be applied for the detection of protein content in activated sludge.

关 键 词：活性污泥 蛋白质检测 考马斯亮蓝法 方法优化 

分 类 号：X830.2[环境科学与工程—环境工程]