高效液相色谱法测定消风止痒颗粒中甘草酸的含量  

Determination of Glycyrrhizic Acid in Xiaofengzhiyang Granules by HPLC

作　　者：甘书明 王树瑶[2] 

机构地区：[1]黑龙江省济仁药业有限公司,哈尔滨150025 [2]北京中医药大学中药学院,北京100102

出　　处：《黑龙江医药》2015年第2期216-217,共2页Heilongjiang Medicine journal

摘　　要：目的:建立HPLC法测定消风止痒颗粒中甘草酸的含量。方法:Diamonsil-C18色谱柱(150mm×4.6mm,5μm);流动相为甲醇-0.2mol/L醋酸铵溶液-冰醋酸(67:33:1);检测波长为250nm。结果:线性范围为44.8μg/ml^448μg/ml,平均回收率为99.5%,RSD为1.2%(n=6)。结论:该方法简便,快速,准确可靠。Objective：A HPLC method was established for the determination of glycyrrhizic acid in Xiaofengzhiyang Granules.Methods： In the analysis,a Diamonsil-C18（150mm×4.6mm,5μm） column,a mobile phase of methanol-0.2mol/L ammonium acetate solution- Glacial acetic acid（67：33：1）and a UV wavelength of 250 nm. Results： A linear range of 44.8μg/ml^448μg/ml,an average recovery of 99.5% with RSD of 1.2%（n=6）. Conclusion： The method of HPLC is simple,rapid and reliable.

关 键 词：高效液相色谱法 甘草酸 消风止痒颗粒 

分 类 号：R927.2[医药卫生—药学]