苦参总黄酮HPLC指纹图谱研究  

Study on HPLC Fingerprint of Flavonoids in Sophora Flavescens Extract

作　　者：刘明玉[1] 单柏松 张凤娇[1] 杨志欣[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《黑龙江医药》2015年第2期227-231,共5页Heilongjiang Medicine journal

基　　金：黑龙江中医药大学"优秀创新人才支持计划"项目(2012);黑龙江省自然科学基金(No.D200744);黑龙江省自然科学基金(No.D200627);黑龙江省教育厅骨干教师资助项目(No.1251G058)

摘　　要：目的:建立苦参提取物黄酮类成分的HPLC指纹图谱,为苦参的质量控制提供依据。方法:采用高效液相色谱法,色谱柱为DIKMA C18柱(250mm×4.6mm,粒度5μm);流动相为甲醇-水(梯度洗脱);流速1ml·min-1;柱温30℃;检测波长280nm;进样量20μl。利用中药色谱指纹图谱相似度评价系统(2004A版)对不同批次苦参总黄酮的HPLC图谱进行比较分析。结果:建立了苦参提取物黄酮类成分的HPLC指纹图谱,以苦参酮为参照峰,对10批苦参总黄酮成分进行指纹图谱分析,标定了13个共有峰,10批提取物的相似度均>0.9。结论:本研究所建立的分析方法具有良好的精密度和重现性,可为苦参提取物的品质评价和质量控制提供科学依据。Objective： To establish the HPLC fingerprint of the flavonoids of Sophora flavescens extract, provide the basis for the quality control of total flavonoids in Sophora Flavescens. Method： Using high performance liquid chromatograhy, The chromatographic column was DIKMA C18column（250mm×4.6mm, particle size 5μm）;The mobile phase was methanol-water（gradient elution）;velocity：1ml-min-1; The column temperature was set at 30℃.The detection wavelength of 280nm; sample size of 20μl.We utilize the similarity evaluation system for chromatographic fingerprint of TCM（version 2004A） to compare and analyze the HPLC of different batchs of total flavonoids in Sophora Flavescens. Results： The HPLC fingerprint of the flavonoids of Sophora flavescens extract was established, with kurainone as the reference peak, the fingerprint analysis of 10 batches of total flavone component, determined 13 common peaks, the 10 batch of extract the similarity of all 0.9. Conclusion： The analysis method developed in this study has good precision and reproducibility, which can provide scientific basis for quality evaluation of radix Sophorae Flavescentis extract and quality control.

关 键 词：苦参提取物 黄酮类成分 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]