响应面法优化超声提取食药用猴头菇多糖的工艺研究  

Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Food Medicinal Hericium by Response Surface Method

作　　者：杨凤杰[1] 沈素媚[1] 冯文婕[1] 

机构地区：[1]浙江经贸职业技术学院,浙江杭州310018

出　　处：《山东化工》2015年第8期22-27,共6页Shandong Chemical Industry

基　　金：浙江经贸职业技术学院科研课题项目(14YB01)

摘　　要：利用响应面分析法研究食药用猴头菇多糖的超声提取工艺。在单因素实验的基础上,采用响应面分析法,以猴头菇多糖产率为响应值优化超声提取工艺,并将最佳条件下猴头菇多糖的超声提取率与水提回流法进行对比。结果表明:猴头菇多糖的最佳超声提取工艺条件:提取温度为60℃,超声时间为35min,液料比为20:1,提取次数2次,提取率为7.48%,比水提回流法提取率高出22.22%;所得模型方程拟合度较好,方法可靠,为超声提取猴头菇多糖工艺提供了一定的理论依据。The technology of ultrasonic- assisted extraction process of polysaccharides from food medicinal hericium was investigated with response surface methodology. On the basis of a series of one - factor experiments, using response surface value, the optimum ultrasonic - assisted extraction process of hericium polysaccharides were determined as the response value, and compared with water extraction reflux method under optimal conditions. Results indicate： 60 of extraction temperature ,35min of ultrasonic time and 20 ： 1 （ mL · g-l ） of liquid - to - solid ratio, and the extraction efficiency of polysaccharides was up to 7.48% under the optimized conditions and was higher 22.22% by water extraction reflux. The fit of the established model was good, the method was reliable, and provided certain theoretical basis by ultrasonic - assisted extraction process of herieium polysaccharides.

关 键 词：响应面分析法 超声提取 猴头菇多糖 

分 类 号：TQ914.1[化学工程]