基于朱顶红愈伤组织途径诱导形成原球茎的研究  

Research on the Protocorm Inducer fron Callus of Hippeastrum vittatum

作　　者：希吉日 田欣 金牧兰 何炎红[2] 田有亮[2] 

机构地区：[1]内蒙古呼和浩特市第二中学,内蒙古呼和浩特010090 [2]内蒙古农业大学,内蒙古呼和浩特010019

出　　处：《安徽农业科学》2015年第13期51-54,共4页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自治区科学技术厅项目(20130438)

摘　　要：[目的]研究通过愈伤组织途径诱导形成朱顶红原球茎。[方法]以朱顶红（Hippeaztrum vittatum）鳞茎作为外植体，通过愈伤组织途径诱导形成原球茎，并建立再生植株。[结果]诱导愈伤组织最佳培养基为MS＋6-BA2．0mg／L＋NAAl．0mg／L，诱导率达到80％；诱导原球茎增殖的培养基为MS＋6-BA1．5mg／L＋NAA0．1mg／L，诱导原球茎系数达到15～20个；诱导原球茎生长最佳培养基为1／2MS＋6-BA4．0mg／L＋NAA0．1ms／L＋KH2PO480mg／L＋TIBA0．5mg／L；诱导原球茎生根培养基为1／2MS＋IBA0．5mg／L，生根率可达100％。[结论]建立了朱顶红再生植株，实现了朱顶红快速繁殖目标，为朱顶红的种苗生产提供技术支撑。[ Objective ] The aim was to study the development of Hippeastrum vittatum by callus induced using bulbs as explants. [ Method ] Cal- lus were induced using bulbs as explants then the protocorm was developed and regeneration plants were established. [ Result] The results showed that the optimal medium for callus induction was MS ＋ 6-BA 2.0 mg/L ＋ NAA 1.0 mg/L, the callus induction rate of bulbs was the highest, the induction rate reached 80% ; medium to induce proliferation of protocorm like bodies was MS ＋6-BA 1.5 mg/L ＋ NAA 0.1 mg/L, inducing pro- tocorm coefficient could reach 15 - 20 ; the optimal medium of protocorm growth was 1/2 MS ＋ 6-BA 4.0 mg/L ＋ NAA 0.1 mg/L ＋ KH2PO4 80 mg,/L ＋ TIBA 0.5 mg/L;Induction of protocorm rooting medium was 1/2 MS ＋ IBA 0. 5mg/L, the rooting rate was up to 100%. [ Conclusion] Regeneration plant of Hippeastrum vittatum was established, and realize the fast breeding goal, in order to provide technical support for seedlings production.

关 键 词：朱顶红 愈伤组织 原球茎 再生植株 

分 类 号：S682.25[农业科学—观赏园艺]