远志炮制前后5种寡糖酯类成分的变化规律  

Variation of Five Oligosaccharide Esters in Polygalae Radix Before and After Processing

作　　者：孟艳[1] 张学兰[1] 唐玉秋[2] 李慧芬[1] 

机构地区：[1]山东中医药大学,济南250355 [2]济南市中心医院,济南250033

出　　处：《中国实验方剂学杂志》2015年第9期10-13,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-013);济南市科技计划项目(201201036)

摘　　要：目的：比较远志不同炮制品中5种寡糖酯类成分的含量,探讨远志炮制前后寡糖酯类成分的变化规律。方法：采用HPLC同时测定远志不同炮制品中西伯利亚远志糖A5,西伯利亚远志糖A6,球腺糖A,tenuifoliside B及3,6＇-二芥子酰基蔗糖的含量,流动相乙腈（A）-0.1%甲酸溶液（B）梯度洗脱（0~5 min,10%~12%A;5~20 min,12%~25%A;20~25 min,25%A）,检测波长330 nm。结果：远志根皮中5种寡糖酯类成分含量均明显高于木心。远志根皮中5个成分质量分数分别为0.173%,0.155%,0.071%,0.120%,0.648%,木心中5个成分质量分数分别为0.090%,0.053%,0.029%,0.018%,0.205%。蜜远志中西伯利亚远志糖A6和球腺糖A含量高于生品,而其他3种成分的含量则较生品降低。制远志中西伯利亚远志糖A5,西伯利亚远志糖A6,3,6＇-二芥子酰基蔗糖和tenuifoliside B含量较生品显著降低,而球腺糖A含量则较生品升高。结论：远志木心与远志根皮中5种寡糖酯类成分含量相差较大,炮制对远志中寡糖酯类成分的含量具有一定影响,经甘草汁煮制后西伯利亚远志糖A5,西伯利亚远志糖A6,3,6＇-二芥子酰基蔗糖及tenuifoliside B均产生了质变。Objective： To compare contents of five kinds of oligosaccharide esters in different processed products of Polygalae Radix and to discuss its variation before and after processing. Method ： Contents of sibiricose A5, sibiricose A6, glomeratose A, tenuifoliside B and 3, 6＇-disinapoyl sucrose in different processed products of Polygalae Radix were determined by HPLC, mobile phase of acetonitrile-0. 1% formic acid solution in gradient program and detection wavelength of 330 nm. Result： Contents of five oligosaccharide esters in root bark of Polygalae Radix were all obviously higher than them in the core. Contents of these five ingredients in root bark were 0.173%, 0.155%, 0.071%, 0.120%, 0.648%, contents of them in the core were 0.090%, 0. 053% , 0. 029% , 0. 018% , 0. 205% , respectively. In honey-stir-baked products, contents of sibiricose A6 and glomeratose A were higher than them in the crude, but sibiricose A5, tenuifoliside B and 3, 6＇-disinapoyl sucrose were lower than them in the crude. In licorice juice boiled products, contents of sibiricose A5 , sibiricose A6 , tenuifoliside B and 3, 6＇-disinapoyl sucrose were obviously lower than them in the crude, but glomeratose A was higher than them in the crude. Conclusion： Contents of five oligosaccharide esters are quite different between root bark and the core of Polygalae Radix, processing has a certain influence on contents of oligosaccharide esters. Sibiricose A5, sibiricose A6, tenuifoliside B and 3, 6＇-disinapoyl sucrose in Polygalae Radix all have qualiy change after boiled with licorice juice.

关 键 词：远志 炮制品 寡糖酯类 球腺糖A 西伯利亚远志糖A5 

分 类 号：R943.1[医药卫生—药剂学] R283.3[医药卫生—药学]