麻杏二陈汤煮散质量标准  

Quality Standard for Boiled Powder of Maxing Erchen Tang

作　　者：刘倩[1] 陈金月[1] 文隽[1] 蓝传炎 

机构地区：[1]广西中医药大学第一附属医院,南宁530023

出　　处：《中国实验方剂学杂志》2015年第9期71-73,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区卫生厅科研课题(Z2012162)

摘　　要：目的：建立麻杏二陈汤煮散质量标准。方法：采用薄层色谱法（TLC）对煮散处方中的炙麻黄、苦杏仁、射干、陈皮进行定性鉴别,采用高效液相色谱法（HPLC）对煮散中的射干苷和橙皮苷含量进行同时测定。结果：TLC法能定性检出炙麻黄、苦杏仁、射干、陈皮,斑点清晰,且阴性对照无干扰。HPLC法测定射干苷在12.0~120.0 mg·L^-1呈良好的线性关系（r=0.999 8）,平均回收率100.00%（RSD 1.9%）;橙皮苷在6.6~66.0 mg·L^-1呈良好的线性关系（r=0.999 9）,平均回收率98.16%（RSD 1.8%）。结论：该研究所建立的定性、定量检测方法操作简便,结果准确可靠,重复性好,能有效地控制麻杏二陈汤煮散的质量。Objective： To establish the quality standard for boiled powder of Maxing Erchen Tang. Method： The qualitative identifications of honey-fried Ephedrae Herba, Armeniacae Semen Amarum, Belamcandae Rhizoma and Citri Reticulatae Pericarpium were carried out by TLC, and the contents of belamcandin and hesperidin were determined by HPLC. Result： The results of TLC showed that the relevant spots were clear without interference in the blank reference. The liner ranges of belamcandin and hesperidin were 12.0-120.0 mg·L^-1（ r = 0. 999 8 ） and 6.6-66.0 mg·L^-1 （ r = 0. 999 9） , respectively. The average recovery of belamcandin was 100.00% with RSD of 1.9%. The average recovery of hesperidin was 98.16% with RSD of 1.8%. Conclusion： The established qualitative and quantitative methods are simple and accurate, reliable and reproducible, and can be effectively used for the quality control of boiled powder of Maxing Erchen Tang.

关 键 词：麻杏二陈汤煮散 薄层色谱法 高效液相色谱法 射干苷 橙皮苷 

分 类 号：R284.1[医药卫生—中药学]