砂仁挥发油抗炎活性谱效关系研究  

Correlation Analysis Between GC-MS Fingerprint of Essential Oil of Amomi Fructus and Anti-inflammatory Activity

作　　者：李生茂[1] 曾滨阳 叶强[3] 敖慧[3] 李鸿翔[3] 

机构地区：[1]川北医学院药学院,四川南充637000 [2]武警辽宁省总队医院,沈阳110034 [3]成都中医药大学,成都611137

出　　处：《中国实验方剂学杂志》2015年第9期133-136,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省中医药管理局青年中医药课题(2014K090);成都中医药大学科技发展基金项(ZRVI5201271);成都中医药大学实验技术项(13117)

摘　　要：目的：研究7批不同产地砂仁挥发油气象色谱-质谱联用（GC-MS）指纹图谱与抗炎活性的关系,为揭示砂仁挥发油抗炎物质基础提供依据。方法：7批不同产地的砂仁分别为广西、吴川、蟠龙、春城、永宁的阳春砂,云南、缅甸的绿壳砂,采用GC-MS建立不同产地砂仁挥发油指纹图谱。取昆明种小鼠90只,随机分为9组,分别为模型组,地塞米松组（12.5 mg·kg^-1）,广西组、吴川组、蟠龙组、春城组、永宁组、云南组、缅甸组（均为3.2 g·kg^-1的砂仁挥发油）,每组10只,ig给予相应剂量药物,模型组给予等体积的生理盐水,每天1次,连续ig 4 d,经二甲苯造模,测定耳肿胀。数据统计分析后,采用灰色关联度分析法研究谱效关系。结果：建立7批不同产地砂仁挥发油GC-MS指纹图谱,确定了其中13个色谱峰为共有峰,并对各共有峰进行了化学成分鉴定;7批砂仁挥发油均有不同程度的抗炎作用,与模型组比较,广西和春城的砂仁无明显的抗炎作用,其他产地和地塞米松均有明显的抗炎作用（P〈0.05,P〈0.01）。13个共有峰与抗炎活性均存在一定的关联度,关联度在0.857~0.734,关联度大小依次为：龙脑〉芳樟醇〉樟脑〉α-松油醇〉α-香柠烯醇〉D-柠檬烯〉乙酸龙脑酯〉α-檀香醇〉月桂烯〉匙叶桉油烯醇〉α-杜松醇〉莰烯〉α-蒎烯。结论：砂仁挥发油抗炎作用为“多成分”共同起效的结果,其中抗炎作用较好的吴川和永宁的阳春砂其龙脑、芳樟醇含量均较高,具有明显的关联度。Objective： To study the correlation between gas chromatography-mass spectrometry （GC- MS） fingerprint of the essential oil of Amomi Fructus and anti-inflammatory activity, and to provide a reference for revealing its efficacy material basis of anti-inflammatory. Method： The fingerprints of the essential oil of Amomi Fructus from different habitats （Amomum villosum from Guangxi, Wuchuan, Panlong, Chuncheng and Yongning; A. villosum, xanthioides Wu et Senjen from Yunnan and Myanmar） were established by GC-MS, and anti- inflammatory activity of the essential oil was observed by the ear swelling caused by dimethylbenzene in mice. The 90 mice were randomly divided into the model group, the dexamethasone group （12.5 mg·kg^-1） , the Guangxi, Wuchuan, Panlong, Chuncheng, Yongning, Yunnan and Myanmar groups （3.2 g ·kg^-1） of 10 mice each. The mice in the model group were intragastrically administrated with normal saline and the other mice were intragastrically administrated with the corresponding medicines once daily for 4 days. The correlation between the fingerprint and anti-inflammatory activity was analyzed by grey relational analysis. Result： The GC-MS fingerprint of the essential oil of Amomi Fructus was established from different habitats, and 13 common peaks were identified. Compared with model group, the essential oils from Guangxi and Chuncheng had no obvious anti-inflammatoryactivity, and the others had obvious anti-inflammatory activity as compared to the dexamethasone group （P 〈 0.05, P 〈 0.01 ）. The correlation between GC-MS fingerprint and the anti-inflammatory activity was from 0. 857 -0. 734, with ranking of borneol 〉 linalool 〉 camphor 〉 α-terpineol 〉 α-bergamotenol 〉 D-limonene 〉 bomyl acetate 〉 α- santalol 〉 myrcene 〉 spathulenol 〉 α-juniper alcohol 〉 camphene 〉 α-pinene. Conclusion： The anti-inflammatory activity of the essential oil of Amomi Fructus may be the combined effect of a variety of chemical constituents. There are definite eorre

关 键 词：砂仁挥发油 GC-MS图谱 抗炎活性 谱效关系 

分 类 号：R285.5[医药卫生—中药学]