岩黄连总碱对人肺癌A549细胞增殖,凋亡及Caspase,Survivin表达的影响  

Effect of Corydalis saxicola Total Alkaloids on Human A549 Cell Proliferation,Apoptosis and Expressions of Caspase,Survivin

作　　者：李金花[1] 王绩英[1] 曾锦荣[1] 高远[1] 李蜜蜜[1] 余圆圆[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001

出　　处：《中国实验方剂学杂志》2015年第9期165-169,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅中医药科技专项(GZPT13-46;GZPT14-37;GZLC14-37);广西壮族自治区卫生厅重点课题项目(重2011008)

摘　　要：目的:初步探讨岩黄连总碱对人肺腺癌A549细胞增殖抑制及诱导凋亡的作用,及其可能的作用机制。方法:将肺癌A549细胞株在体外条件下加入不同质量浓度岩黄连总碱。用四甲基偶氮唑蓝比色(MTT)法检测不同浓度(0.1,0.05,0.01,0.005 g·L-1)岩黄连总碱分别作用24,48,72 h后,对人肺腺癌A549细胞的生长抑制情况。采用Hoechst染色法检测岩黄连总碱处理A549细胞48 h的生长情况。流式细胞术检测岩黄连总碱不同药物浓度诱导凋亡的情况。RT-PCR法检测岩黄连及顺铂对A549细胞中Caspase-3,Survivin mRNA的表达影响变化情况。结果:MTT结果提示岩黄连总碱对肺癌A549细胞增殖具有一定的抑制作用,且抑制作用呈现时间-剂量关系。MTT及Hoechst染色显示岩黄连总碱组较阴性组明显抑制A549细胞增殖(P<0.05)。流式细胞仪检测结果显示岩黄连诱导A549细胞凋亡作用随着药物浓度的增加,凋亡率升高。RT-PCR结果提示岩黄连组较阴性组上调了Caspase-3 mRNA的表达,下调了Survivin mRNA的表达。结论:岩黄连总碱具有一定的抑制人肺腺癌A549细胞增殖和诱导凋亡作用,并呈时间-剂量关系。其机制可能与上调Caspase-3的表达和下调Survivin的表达有关。Objective： The aim of this study was to study the impact of total alkaloids of Corydalis saxicola on human lung cancer A549 cell proliferation and apoptosis and to explore its possible mechanism. Method： The logarithmic growth phase of A549 cells were cultured in vitro and incubated with C. saxicola Banting. MTT assay was used to detect the proliferation of A549 cells after the treatment of Corydalis saxicola at different concentrations （0. 1, 0.05, 0.01, 0. 005 g ·L^-1 ） for different time （24, 48, 72 h）. Hoechst stain was applied to analyze the apoptosis effects. Flow cytometry were used to anlyze cell apotosis. The mRNA expression changes of Caspase-3 and Survivin after treatments were detected by RT-PCR. Result： C. saxicola could inhibit the proliferation of A549 cells in dose-time manner. After 48 h, inhibition of C. saxicola group in A549 cells was stronger than the control group （P 〈 0.05 ）. Flow cytometry showed that the apoptotic rate increased with the concentration increased （P 〈 0.05）. RT-PCR results suggested that compared with the control group, C. saxicola group up-regulated the expression of Caspase-3 mRNA and down-regulated the expression of Survivin mRNA （P 〈 0.05）. Conclusion： C. saxicola can inhibit proliferation and induce the apoposis of A549 cells in a time-and dose-dependent manner. The mechanism may be related to the up-regulation of Caspase-3 expression and down-regulation of Survivin expression.

关 键 词：岩黄连 肺癌 增殖 凋亡 Caspase-3 Survivin 

分 类 号：R285.5[医药卫生—中药学]