雷公藤制剂指纹图谱及谱效关系研究  

Fingerprint and spectrum-effect relationships on Tripterygium glycosides preparation

作　　者：池婕 林兵[2] 刘志宏[2] 杨丽娜[2] 刘雪梅[2] 宋洪涛[2] 

机构地区：[1]厦门大学医学院基础医学系,福建厦门361102 [2]厦门大学附属东方医院药学科,福建福州350025

出　　处：《中国中药杂志》2015年第8期1479-1483,共5页China Journal of Chinese Materia Medica

基　　金：福建省科技计划重大项目(201211001)

摘　　要：采用高效液相色谱法(HPLC)建立7个厂家雷公藤多苷片醋酸乙酯提取物的指纹图谱,得到11个共有峰,各厂家指纹峰种类和含量差异较大。选择Con A刺激的小鼠脾淋巴细胞模型进行药效学研究,分别给予30,15,7.5,3.75,1.88,0.94mg·L^(-1)的雷公藤多苷片提取物进行处理,48 h后测定各药物对小鼠脾淋巴细胞增殖的作用,发现各厂家的雷公藤多苷片均对小鼠脾淋巴细胞增殖有显著抑制作用(P<0.05),并呈剂量依赖性,且各厂家雷公藤制剂对小鼠脾淋巴细胞增殖的作用的IC_(50)值差异较大。选择灰色关联度分析法分析指纹图谱与增殖抑制药效数据的关系,发现各指纹峰与药效的关联度均大于0.6,表明雷公藤增殖抑制药效是多成分共同起效的结果,其中5号峰与10号峰对增殖抑制作用贡献最大,各共有峰与药效之间关联序为5>10>8>7>6>11>4>9>2>3>1。该文初步探讨了雷公藤的物质基础,为明确雷公藤的作用机制,建立更加全面可靠的质量控制方法提供理论依据与数据支持。Tripterygium glycosides preparation which extracted from the traditional Chinese herb Tripterygium wilfordii （TWHF）, was widely used to treat the autoimmune diseases. Previous works demonstrated that TWHF had potent anti-inflammatory and immuno- suppressive properties. But the different quality and high incident rate of side effects of different manufactures inhibited its clinical ap- plication. Since TWHF had been generally known to play a therapeutical effect by synergism of multiple constituents, it was necessary to build the relationship between the HPLC fingerprint and bioactivity so as to ensure the quality safety and efficacy. The HPLC finger- print showed that description and content of peaks from different manufactures were diverse. Only 11 common peaks were found. In this study, mice spleen cells stimulated by Con A were used to test the proliferation inhibition bioactivity of TWHF preparations, which were incubated with 30, 15, 7. 5, 3. 75, 1.88 and 0. 94 mg· L-1TWHF preparations for 48 h. The results showed that mice spleen cells proliferation was inhibited by all TWHF preparations significantly compared with the control group, which suggested the TWHF preparations showed immune suppress activity. The TWHF preparations from 7 manufacture showed different IC50 value, which might belong to different contents which showed in the HPLC fingerprint. Moreover, a relationship between the HPLC fingerprint and the bio- activity were established to identify important constituents by grey relational analysis （GRA）. The result showed that all the contents were relative with the IC50, especially No. 5 and 10 peaks, but No. 1 peak, which was proved to be triptolide, had few contribute to the inhibition of mice spleen ceils proliferation. The study of relationship between the HPLC fingerprint and the IC50 by GRA could helpto investigate mechanism of bioactive and provide an evidence for the quantification of multi-constituents.

关 键 词：雷公藤 指纹图谱 细胞增殖 谱效关系 灰色关联度分析 

分 类 号：R285.5[医药卫生—中药学]