狭基线纹香茶菜水溶性总黄酮中6种碳苷黄酮的含量测定和抗肿瘤活性研究  

Determination of six C-Glycoside flavones and antitumor activity of water-soluble total flavonoids from Isodon lophanthoides var.gerardianus

作　　者：张扬[1] 唐海明[1] 黎爱[1] 徐兰芳[1] 陈建南[1] 黄松[1] 贺莲 

机构地区：[1]广州中医药大学新药开发研究中心,广东广州510006 [2]中山大学新华学院,广东东莞523133

出　　处：《中国中药杂志》2015年第8期1543-1547,共5页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2012BAI29B09)

摘　　要：该文建立了HPLC同时测定狭基线纹香茶菜水溶性总黄酮中6种碳苷黄酮含量的方法,并对狭基线纹香茶菜水溶性总黄酮进行抗肿瘤活性评价。采用Kromasil 100-5 C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-乙腈-0.5%甲酸梯度洗脱;体积流量0.8 mL·min^(-1),检测波长334 nm;柱温为室温(25℃)。以HepG2细胞为供试细胞株,采用MTT法评价水溶性总黄酮的抗肿瘤活性。新西兰牡荆苷2、新西兰牡荆苷3、异夏佛塔苷、夏佛塔苷、牡荆苷和芹菜素-6,8-二-C-α-L-吡喃阿拉伯糖苷线性范围分别为0.25~2.53,0.12~1.20,0.37~3.69,0.16~1.63,0.19~1.92,0.14~1.42μg;平均回收率(n=6)分别为99.6%,100.2%,99.6%,97.9%,98.8%,98.6%,RSD分别为0.87%,2.0%,1.8%,1.5%,1.2%,1.2%。以IC_(50)值作为评价指标,测得狭基线纹香共菜水溶性总黄酮在给药24,48,72 h后抑制HepG2细胞增殖的IC_(50)值分别为1.89,1.71,1.51g·L^(-1)。本法快速、简便、准确,重复性好,可用于狭基线纹香共菜水溶性总黄酮的质量控制。狭基线纹香茶菜水溶性总黄酮对HepG2细胞增殖有一定的抑制作用。This research established an HPLC method for determination of six C-Glycoside flavones of warer-soluble total flavonoids from Isodon lophanthoides vat. gerardianus （Benth.）H. Hara, and studied the antitumor activity of the warer-soluble total flavonoids. The HPLC system consisted of Kromasil 100-5 C18（4.6 mm × 250 mm, 5 μm）column and a solution system of methanol, aeetonitrile and 0. 5% formic acid gradient elution at a flow rate of 0. 8 mL · min-1 and the wavelength of detector was at 334 nm. The column temperature was 25 ℃. The antitumor activity of water-soluble flavonoids was assayed using HepG2 cell as the tested cell. The linear ranges of vicenin Ⅱ , vicenin Ⅲ, isoschaftoside, sehaftoside, vitexin, 6, 8-di-C-α-L-arabinosylapigenin were 0. 25-2. 53, 0. 12-1.20, 0. 37-3.69, 0. 16-1.63, 0. 19-1.92, 0. 14-1.42 μg, respectively. The average recoveries （ n = 6） were 99. 6% （ RSD 0. 87% ）, 100. 2% （RSD 2. 0% ）, 99. 6% （ RSD 1.8% ）, 97.9% （RSD 1.5% ）, 98. 8% （RSD 1.2% ）, 98. 6% （ RSD 1.2% ）, respective- ly. After exposure in 24, 48, 72 h, the total flavonoids showed inhibitory effect on the proliferation of HepG2 ceils with IC50 as the evaluation index, the IC50 values of 1.89,1.71, 1.51 g · L-1 , respectively. The method is quick, simple and accurate with good＇re-producibility, and can be used for determination of vicenin Ⅱ, viceninⅢ, isoschaftoside, schaftoside, vitexin, 6, 8-di-C-α-L-arabino- sylapigenin in the warer-soluble total flavonoids from I. Iophanthoides var. gerardianus. The warer-soluble total flavonoids from I. loph- anthoides have inhibitory effect on the proliferation of HepG2 cells.

关 键 词：狭基线纹香茶菜 水溶性黄酮 高效液相色谱法 HEPG2 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]