3种枝状地衣乙酸乙酯提取液对两性绵霉的抑菌效果  

Fungistasis Effects of Ethyl Acetate Extracts from 3 Fruticose Lichens on Achyla bisexalis

作　　者：刘文霞[1] 郭守玉[2] 陈家长[3] 韩留福[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050024 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]中国水产科学研究院淡水渔业研究中心,无锡214081

出　　处：《菌物研究》2015年第1期20-23,共4页Journal of Fungal Research

基　　金：国家罗非鱼产业技术体系养殖技术与环境专项(CARS-49);小五台山国家级自然保护区地衣生态学研究(C2012205009)

摘　　要：研究了3种枝状地衣乙酸乙酯提取液对水霉病主要病原菌两性绵霉菌丝的体外抑菌效果。菌株分离自河流水体,经培养、形态观察及ITS区序列测定比对,鉴定为两性绵霉Achyla bisexalis。结果显示枝状地衣黑穗石蕊、中国树花、地茶的乙酸乙酯浸提液均对两性绵霉有抑制作用,最低抑制浓度均为800 mg/L;在供试浓度为3 200 mg/L时,3种地衣提取物均能完全抑制菌丝的生长。The inhibiting effects of ethyl acetate extracts from 3 fruticose lichens on the mycilia of Achyla bisexalis which can cause serious saprolegniasis were investigated. The strain of Achyla bisexalis was isolated from river,and identified based on the morphological characters and ITS r DNA sequence alignment. The results demonstrated that the extracts from tested dried lichen samples could inhibit the growth of mycelia of Achyla bisexalis. The minimum inhibitory concentration was 800 mg /L for all 3 lichen species Cladonia amaurocraea,Ramalina sinensis and Thamnolia vermicularis. Furthermore,the results also showed that the Achyla mycelia could be eliminated by the lichen extracts with the concentration up to 3 200 mg / L.

关 键 词：枝状地衣 乙酸乙酯 两性绵霉 抑菌作用 

分 类 号：R285[医药卫生—中药学] Q949.34[医药卫生—中医学]