硫酸化茯苓多糖对小鼠腹腔巨噬细胞功能的影响  

Effects of sulfation pachymaran on the function of mouse peritoneal macrophages

作　　者：高贵珍[1] 孙冰清[1] 吴超[1] 刘小阳[1] 

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《安徽工程大学学报》2015年第1期1-5,共5页Journal of Anhui Polytechnic University

基　　金：安徽省教育厅自然科学基金资助项目(KJ2013A242)

摘　　要：研究硫酸化茯苓多糖（SP）对脂多糖（LPS）诱导的体内外小鼠巨噬细胞氧化损伤的影响.体外实验中,制备小鼠腹腔巨噬细胞,加入不同质量浓度（0~100mg/mL）的SP和1mg/mL的LPS诱导剂,24h后检测细胞活力、一氧化氮（NO）、超氧阴离子自由基（O-2）及肿瘤坏死因子-α（TNF-α）含量.体内实验中,42只ICR小鼠随机分为空白组、模型组和预防组,采用腹腔注射法连续给药7天后收集腹腔巨噬细胞,检测多种生化指标.实验结果表明：LPS可降低巨噬细胞的活性,增加O-2的释放和NO、TNF-α的分泌;SP能够显著降低NO的水平,提高巨噬细胞抗O-2的活力,对于TNF-α的含量没有显著影响.SP可有效地改善LPS对小鼠腹腔巨噬细胞的氧化损伤作用.The effects of sulfation pachymaran（SP）on the oxidative damage of murine peritoneal macrophages induced by lipopolysaccharide（LPS）in vitro and vivo models were investigated.In vitro experiment,the peritoneal macrophages were harvested from ICR mice and then treated with 1mg/mL of LPS and 0~100mg/mL of SP.The cell activity,nitric oxide（NO）,superoxide anion redical（O-2）and tumor necrosis factor-α（TNF-α）were assayed respectively after 24hours＇ cultivation.In vivo experiment,42 female ICR mice were randomly divided into control group,prevention group and LPS group.After successive administration of mice by intraperitoneal injection for 7days,peritoneal macrophages were collected to detect different biochemical indexes.LPS reduced decreased cell viability of macrophages,increased the release of O-2,NO,and TNF-α;SP significantly reduced the production of O-2and NO,No significant effects were observed on the release of TNF-α.SP effectively meliorates the oxidative damage of murine peritoneal macrophages induced by LPS.

关 键 词：硫酸化茯苓多糖 脂多糖 巨噬细胞 氧化活性 

分 类 号：R285.5[医药卫生—中药学]